Skip to main content
placeholder image

High-dimensional and spatial analysis reveals immune landscape dependent progression in cutaneous squamous cell carcinoma.

Journal Article


Abstract


  • Purpose

    The tumour immune microenvironment impacts the biological behaviour of the tumour but its effect on clinical outcomes in head and neck cutaneous squamous cell carcinomas (HNcSCC) is largely unknown.

    Experimental design

    We compared the immune milieu of high-risk HNcSCC that never progressed to metastasis with those that metastasised using multi-parameter imaging mass cytometry. The cohort included both immunosuppressed patients (IS) and patients with an absence of clinical immune-suppression (ACIS). Spatial analyses were used to identify cellular interactions that were associated with tumour behaviour.

    Results

    Non-progressing primary HNcSCC were characterised by higher CD8+ and CD4+ T cell responses, including numerically increased Regulatory T cells. By contrast, primary lesions from HNcSCC patients who progressed were largely devoid of T cells with lower numbers of innate immune cells and increased expression of checkpoint receptors and in the metastatic lesions were characterised by an accumulation of B cells. Spatial analysis reveals multiple cellular interactions associated with non-progressing primary tumours that were distinct in primary tumours of disease progressing patients. Cellular regional analysis of the tumour microenvironment also shows squamous cell-enriched tumour regions associated with primary non-progressing tumours.

    Conclusions

    Effective responses from both CD8+ and CD4+ T cells in the tumour microenvironment are essential for immune control of primary HNcSCC. Our findings indicate that the early events that shape the immune responses in primary tumours dictate progression and disease outcomes in HNcSCC.

Publication Date


  • 2022

Citation


  • Ferguson, A. L., Sharman, A. R., Allen, R. O., Ye, T., Lee, J. H., Low, T. -H. H., . . . Palendira, U. (2022). High-dimensional and spatial analysis reveals immune landscape dependent progression in cutaneous squamous cell carcinoma.. Clinical cancer research : an official journal of the American Association for Cancer Research, CCR-22-1332. doi:10.1158/1078-0432.ccr-22-1332

Web Of Science Accession Number


Start Page


  • CCR-22-1332

Abstract


  • Purpose

    The tumour immune microenvironment impacts the biological behaviour of the tumour but its effect on clinical outcomes in head and neck cutaneous squamous cell carcinomas (HNcSCC) is largely unknown.

    Experimental design

    We compared the immune milieu of high-risk HNcSCC that never progressed to metastasis with those that metastasised using multi-parameter imaging mass cytometry. The cohort included both immunosuppressed patients (IS) and patients with an absence of clinical immune-suppression (ACIS). Spatial analyses were used to identify cellular interactions that were associated with tumour behaviour.

    Results

    Non-progressing primary HNcSCC were characterised by higher CD8+ and CD4+ T cell responses, including numerically increased Regulatory T cells. By contrast, primary lesions from HNcSCC patients who progressed were largely devoid of T cells with lower numbers of innate immune cells and increased expression of checkpoint receptors and in the metastatic lesions were characterised by an accumulation of B cells. Spatial analysis reveals multiple cellular interactions associated with non-progressing primary tumours that were distinct in primary tumours of disease progressing patients. Cellular regional analysis of the tumour microenvironment also shows squamous cell-enriched tumour regions associated with primary non-progressing tumours.

    Conclusions

    Effective responses from both CD8+ and CD4+ T cells in the tumour microenvironment are essential for immune control of primary HNcSCC. Our findings indicate that the early events that shape the immune responses in primary tumours dictate progression and disease outcomes in HNcSCC.

Publication Date


  • 2022

Citation


  • Ferguson, A. L., Sharman, A. R., Allen, R. O., Ye, T., Lee, J. H., Low, T. -H. H., . . . Palendira, U. (2022). High-dimensional and spatial analysis reveals immune landscape dependent progression in cutaneous squamous cell carcinoma.. Clinical cancer research : an official journal of the American Association for Cancer Research, CCR-22-1332. doi:10.1158/1078-0432.ccr-22-1332

Web Of Science Accession Number


Start Page


  • CCR-22-1332