Five NH-derivatives of ciprofloxacin (2-6) have been prepared by acetylation/ benzoylation on ciprofloxacin (1) in order to carry out screening for antibacterial activities on some gram-positive and gram-negative bacteria. The antifungal activity of these compounds has also been made against Candida albicans. All of the derivatives have shown enhanced activities against gramnegatives bacteria than parent antibiotic, ciprofloxacin among which compounds 2 and 6 are the most potent agents. Regarding the antifungal activity all of the compounds have shown highest activity than ciprofloxacin. All the compounds have been characterized with spectral analysis.