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Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola

Journal Article


Abstract


  • Canola varieties exhibit variation in drought avoidance and drought escape traits, reflecting adaptation to water-deficit environments. Our understanding of underlying genes and their interaction across environments in improving crop productivity is limited. A doubled haploid��population was analysed to identify quantitative trait loci��(QTL) associated with water-use efficiency (WUE) related traits. High WUE in the vegetative phase was associated with low seed yield. Based on the resequenced parental genome data, we developed sequence-capture-based markers and validated their linkage with carbon isotope discrimination (��13C) in an F2 population. RNA��sequencing was performed to determine the expression of candidate genes underlying ��13C QTL. QTL contributing to main and QTL �� environment interaction effects for ��13C and yield were identified. One multiple-trait QTL for ��13C, days to flower, plant height, and seed yield was identified on chromosome A09. Interestingly, this QTL region overlapped with a homoeologous exchange��(HE) event, suggesting its association with the multiple traits. Transcriptome analysis revealed 121 significantly differentially expressed genes underlying ��13C QTL on A09 and C09, including in HE regions. Sorting out the negative relationship��between vegetative WUE and seed yield is a priority. Genetic and genomic resources and knowledge so developed could improve canola WUE and yield.

Publication Date


  • 2022

Citation


  • Raman, H., Raman, R., Pirathiban, R., McVittie, B., Sharma, N., Liu, S., . . . Zhang, Y. (2022). Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola. Plant Cell and Environment, 45(7), 2019-2036. doi:10.1111/pce.14337

Scopus Eid


  • 2-s2.0-85129372261

Start Page


  • 2019

End Page


  • 2036

Volume


  • 45

Issue


  • 7

Place Of Publication


Abstract


  • Canola varieties exhibit variation in drought avoidance and drought escape traits, reflecting adaptation to water-deficit environments. Our understanding of underlying genes and their interaction across environments in improving crop productivity is limited. A doubled haploid��population was analysed to identify quantitative trait loci��(QTL) associated with water-use efficiency (WUE) related traits. High WUE in the vegetative phase was associated with low seed yield. Based on the resequenced parental genome data, we developed sequence-capture-based markers and validated their linkage with carbon isotope discrimination (��13C) in an F2 population. RNA��sequencing was performed to determine the expression of candidate genes underlying ��13C QTL. QTL contributing to main and QTL �� environment interaction effects for ��13C and yield were identified. One multiple-trait QTL for ��13C, days to flower, plant height, and seed yield was identified on chromosome A09. Interestingly, this QTL region overlapped with a homoeologous exchange��(HE) event, suggesting its association with the multiple traits. Transcriptome analysis revealed 121 significantly differentially expressed genes underlying ��13C QTL on A09 and C09, including in HE regions. Sorting out the negative relationship��between vegetative WUE and seed yield is a priority. Genetic and genomic resources and knowledge so developed could improve canola WUE and yield.

Publication Date


  • 2022

Citation


  • Raman, H., Raman, R., Pirathiban, R., McVittie, B., Sharma, N., Liu, S., . . . Zhang, Y. (2022). Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola. Plant Cell and Environment, 45(7), 2019-2036. doi:10.1111/pce.14337

Scopus Eid


  • 2-s2.0-85129372261

Start Page


  • 2019

End Page


  • 2036

Volume


  • 45

Issue


  • 7

Place Of Publication