Skip to main content
placeholder image

Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation

Journal Article


Abstract


  • Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2 (NGN2) has been widely used to generate different populations of neurons, which have been used in neurodevelopment studies, disease modeling, drug screening, and neuronal replacement therapies. Could NGN2 be a “one-glove-fits-all” approach for neuronal differentiations? This review summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for the rapid and directed differentiation of neurons.

Publication Date


  • 2022

Citation


  • Hulme, A. J., Maksour, S., St-Clair Glover, M., Miellet, S., & Dottori, M. (2022). Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation. Stem Cell Reports, 17(1), 14-34. doi:10.1016/j.stemcr.2021.11.015

Scopus Eid


  • 2-s2.0-85122229089

Start Page


  • 14

End Page


  • 34

Volume


  • 17

Issue


  • 1

Abstract


  • Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2 (NGN2) has been widely used to generate different populations of neurons, which have been used in neurodevelopment studies, disease modeling, drug screening, and neuronal replacement therapies. Could NGN2 be a “one-glove-fits-all” approach for neuronal differentiations? This review summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for the rapid and directed differentiation of neurons.

Publication Date


  • 2022

Citation


  • Hulme, A. J., Maksour, S., St-Clair Glover, M., Miellet, S., & Dottori, M. (2022). Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation. Stem Cell Reports, 17(1), 14-34. doi:10.1016/j.stemcr.2021.11.015

Scopus Eid


  • 2-s2.0-85122229089

Start Page


  • 14

End Page


  • 34

Volume


  • 17

Issue


  • 1