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A search for genetic factors contributing to nicotine dependence

Journal Article


Abstract


  • Recent research suggests that genetic factors play an important role in conferring susceptibility to nicotine dependence. In order to search for underlying genes we have been carrying out a quantitative trait locus (QTL) approach in mice in combination with association studies in humans. Inbred strains of mice with nicotine preference have been crossed with nicotine avoiding strains (two bottle choice paradigm) and the F1 generation inter-crossed to yield 497 mice of the F2 generation. A group of 50 mice with high preferring phenotypes has been tested for association with polymorphic DNA markers and compared to a group of 50 low preferring mice. Pro-visional QTL's for nicotine preference are being investigated. These mouse QTL's will be tested in homologous regions of the human genome. In addition, a sample of 97 simplex families of smokers with nicotine dependence (triads) have been collected for family based association studies with the corresponding mouse loci. The sample is being used also for the study of candidate genes (dopamine-, serotonin-, acetylcholine-, opioid receptors and -transporters), which may play a role in the development of nicotine dependence. Localization of genes, which confer susceptibility to nicotine dependence, and their subsequent identification would be a first step towards characterization of genetic influences in addiction to smoking. The work has been supported by a grant from VERUM.

Publication Date


  • 2000

Citation


  • Wildenauer, D. B., Aschoff, S., Schwab, S. G., Schmitz, T., Schroff, K. C., Gabel, S., . . . Richter, E. (2000). A search for genetic factors contributing to nicotine dependence. American Journal of Medical Genetics - Neuropsychiatric Genetics, 96(4), 464.

Scopus Eid


  • 2-s2.0-33749105109

Web Of Science Accession Number


Start Page


  • 464

Volume


  • 96

Issue


  • 4

Abstract


  • Recent research suggests that genetic factors play an important role in conferring susceptibility to nicotine dependence. In order to search for underlying genes we have been carrying out a quantitative trait locus (QTL) approach in mice in combination with association studies in humans. Inbred strains of mice with nicotine preference have been crossed with nicotine avoiding strains (two bottle choice paradigm) and the F1 generation inter-crossed to yield 497 mice of the F2 generation. A group of 50 mice with high preferring phenotypes has been tested for association with polymorphic DNA markers and compared to a group of 50 low preferring mice. Pro-visional QTL's for nicotine preference are being investigated. These mouse QTL's will be tested in homologous regions of the human genome. In addition, a sample of 97 simplex families of smokers with nicotine dependence (triads) have been collected for family based association studies with the corresponding mouse loci. The sample is being used also for the study of candidate genes (dopamine-, serotonin-, acetylcholine-, opioid receptors and -transporters), which may play a role in the development of nicotine dependence. Localization of genes, which confer susceptibility to nicotine dependence, and their subsequent identification would be a first step towards characterization of genetic influences in addiction to smoking. The work has been supported by a grant from VERUM.

Publication Date


  • 2000

Citation


  • Wildenauer, D. B., Aschoff, S., Schwab, S. G., Schmitz, T., Schroff, K. C., Gabel, S., . . . Richter, E. (2000). A search for genetic factors contributing to nicotine dependence. American Journal of Medical Genetics - Neuropsychiatric Genetics, 96(4), 464.

Scopus Eid


  • 2-s2.0-33749105109

Web Of Science Accession Number


Start Page


  • 464

Volume


  • 96

Issue


  • 4