Skip to main content
placeholder image

Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells

Journal Article


Abstract


  • A gene encoding a novel RhoGAP of 1146 amino acids was isolated from rat testis RNA. Analysis of this protein identified two conserved domains, a RhoGAP domain and an RA domain. Thus the gene was named RARhoGAP. The RhoGAP domain contained conserved residues critical for RhoGAP activity, suggesting this domain is involved in the down-regulation of Rho GTPases. The presence of the RA domain suggests that RARhoGAP also functions as an effector for Ras- or Ral-like GTPases. RT-PCR analysis showed the transcript was ubiquitous in extragonadal tissues; however, Northern analysis indicated highest expression was in the testis. Homologues of rat RARhoGAP were found in mouse and human and were found expressed in testis by nested RT-PCR. In situ hybridization confirmed the specific expression of RARhoGAP in differentiating male germ cells. We postulate that RARhoGAP may be involved in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis. �� 2004 Elsevier Inc. All rights reserved.

Publication Date


  • 2004

Citation


  • Curry, B. J., Su, H., Law, E. G., McLaughlin, E. A., Nixon, B., & Aitken, R. J. (2004). Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells. Genomics, 84(2), 406-418. doi:10.1016/j.ygeno.2004.03.004

Scopus Eid


  • 2-s2.0-3042561479

Start Page


  • 406

End Page


  • 418

Volume


  • 84

Issue


  • 2

Place Of Publication


Abstract


  • A gene encoding a novel RhoGAP of 1146 amino acids was isolated from rat testis RNA. Analysis of this protein identified two conserved domains, a RhoGAP domain and an RA domain. Thus the gene was named RARhoGAP. The RhoGAP domain contained conserved residues critical for RhoGAP activity, suggesting this domain is involved in the down-regulation of Rho GTPases. The presence of the RA domain suggests that RARhoGAP also functions as an effector for Ras- or Ral-like GTPases. RT-PCR analysis showed the transcript was ubiquitous in extragonadal tissues; however, Northern analysis indicated highest expression was in the testis. Homologues of rat RARhoGAP were found in mouse and human and were found expressed in testis by nested RT-PCR. In situ hybridization confirmed the specific expression of RARhoGAP in differentiating male germ cells. We postulate that RARhoGAP may be involved in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis. �� 2004 Elsevier Inc. All rights reserved.

Publication Date


  • 2004

Citation


  • Curry, B. J., Su, H., Law, E. G., McLaughlin, E. A., Nixon, B., & Aitken, R. J. (2004). Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells. Genomics, 84(2), 406-418. doi:10.1016/j.ygeno.2004.03.004

Scopus Eid


  • 2-s2.0-3042561479

Start Page


  • 406

End Page


  • 418

Volume


  • 84

Issue


  • 2

Place Of Publication