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Do fertilin �� and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins

Journal Article


Abstract


  • Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin �� and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin �� and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.

Publication Date


  • 2001

Citation


  • McLaughlin, E. A., Frayne, J., Bloomerg, G., & Hall, L. (2001). Do fertilin �� and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins. Molecular Human Reproduction, 7(4), 313-317.

Scopus Eid


  • 2-s2.0-0035070684

Start Page


  • 313

End Page


  • 317

Volume


  • 7

Issue


  • 4

Place Of Publication


Abstract


  • Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin �� and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin �� and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.

Publication Date


  • 2001

Citation


  • McLaughlin, E. A., Frayne, J., Bloomerg, G., & Hall, L. (2001). Do fertilin �� and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins. Molecular Human Reproduction, 7(4), 313-317.

Scopus Eid


  • 2-s2.0-0035070684

Start Page


  • 313

End Page


  • 317

Volume


  • 7

Issue


  • 4

Place Of Publication