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Tuneable hybrid hydrogels via complementary self-assembly of a bioactive peptide with a robust polysaccharide

Journal Article


Abstract


  • Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly of low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) and Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous and bioactive hydrogels. Polysaccharides such as agarose are biocompatible, degradable, and non-toxic. Agarose and these Fmoc-peptides have both demonstrated efficacy in vitro and in vivo. These materials have complementary properties; agarose has known mechanics in the physiological range but is inert and would benefit from bioactive and topographical cues found in the fibrous, protein-rich extracellular matrix. Fmoc-DIKVAV and Fmoc-FRGDF are synthetic self-assembling peptides that present bioactive cues "IKVAV"and "RGD"designed from the ECM proteins laminin and fibronectin. The work presented here demonstrates that the addition of agarose to Fmoc-DIKVAV and Fmoc-FRGDF results in physical characteristics that are dependent on agarose concentration. The networks are peptide-dominated at low agarose concentrations, and agarose-dominated at high agarose concentrations, resulting in distinct changes in structural morphology. Interestingly, at mid-range agarose concentration, a hybrid network is formed with structural similarities to both peptide and agarose systems, demonstrating reinforced mechanical properties. Bioactive-LMWG polysaccharide hydrogels demonstrate controllable microenvironmental properties, providing the ability for tissue-specific biomaterial design for tissue engineering and 3D cell culture.

UOW Authors


Publication Date


  • 2021

Citation


  • Firipis, K., Boyd-Moss, M., Long, B., Dekiwadia, C., Hoskin, W., Pirogova, E., . . . Williams, R. J. (2021). Tuneable hybrid hydrogels via complementary self-assembly of a bioactive peptide with a robust polysaccharide. ACS Biomaterials Science and Engineering, 7(7), 3340-3350. doi:10.1021/acsbiomaterials.1c00675

Scopus Eid


  • 2-s2.0-85109017481

Web Of Science Accession Number


Start Page


  • 3340

End Page


  • 3350

Volume


  • 7

Issue


  • 7

Abstract


  • Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly of low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) and Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous and bioactive hydrogels. Polysaccharides such as agarose are biocompatible, degradable, and non-toxic. Agarose and these Fmoc-peptides have both demonstrated efficacy in vitro and in vivo. These materials have complementary properties; agarose has known mechanics in the physiological range but is inert and would benefit from bioactive and topographical cues found in the fibrous, protein-rich extracellular matrix. Fmoc-DIKVAV and Fmoc-FRGDF are synthetic self-assembling peptides that present bioactive cues "IKVAV"and "RGD"designed from the ECM proteins laminin and fibronectin. The work presented here demonstrates that the addition of agarose to Fmoc-DIKVAV and Fmoc-FRGDF results in physical characteristics that are dependent on agarose concentration. The networks are peptide-dominated at low agarose concentrations, and agarose-dominated at high agarose concentrations, resulting in distinct changes in structural morphology. Interestingly, at mid-range agarose concentration, a hybrid network is formed with structural similarities to both peptide and agarose systems, demonstrating reinforced mechanical properties. Bioactive-LMWG polysaccharide hydrogels demonstrate controllable microenvironmental properties, providing the ability for tissue-specific biomaterial design for tissue engineering and 3D cell culture.

UOW Authors


Publication Date


  • 2021

Citation


  • Firipis, K., Boyd-Moss, M., Long, B., Dekiwadia, C., Hoskin, W., Pirogova, E., . . . Williams, R. J. (2021). Tuneable hybrid hydrogels via complementary self-assembly of a bioactive peptide with a robust polysaccharide. ACS Biomaterials Science and Engineering, 7(7), 3340-3350. doi:10.1021/acsbiomaterials.1c00675

Scopus Eid


  • 2-s2.0-85109017481

Web Of Science Accession Number


Start Page


  • 3340

End Page


  • 3350

Volume


  • 7

Issue


  • 7