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Neurodegenerative disease-associated protein aggregates are poor inducers of the heat shock response in neuronal cells.

Journal Article


Abstract


  • Protein aggregates that result in inclusions formation are a pathological hallmark common to many neurodegenerative diseases, including amyotrophic lateral sclerosis, Parkinson's disease and Huntington's disease. Under conditions of cellular stress, activation of the heat shock response (HSR) results in an increase in the levels of molecular chaperones and is a first line of cellular defence against inclusion formation. It remains to be established whether neurodegenerative disease-associated proteins and inclusions are themselves capable of inducing an HSR in neuronal cells. To address this, we generated a neuroblastoma cell line that expresses a fluorescent reporter protein under conditions of heat shock transcription factor 1-mediated HSR induction. We show that the HSR is not induced by exogenous treatment with aggregated forms of recombinant α-synuclein or the G93A mutant of superoxide dismutase-1 (SOD1G93A) nor intracellular expression of SOD1G93A or a pathogenic form of polyQ-expanded huntingtin (Htt72Q). These results suggest that pathogenic proteins evade detection or impair induction of the HSR in neuronal cells. A failure of protein aggregation to induce an HSR may contribute to the development of inclusion pathology in neurodegenerative diseases.

Publication Date


  • 2020

Citation


  • Gil, R. S., Cox, D., McAlary, L., Berg, T., Walker, A. K., Yerbury, J. J., . . . Ecroyd, H. (2020). Neurodegenerative disease-associated protein aggregates are poor inducers of the heat shock response in neuronal cells.. J Cell Sci. doi:10.1242/jcs.243709

Web Of Science Accession Number


Abstract


  • Protein aggregates that result in inclusions formation are a pathological hallmark common to many neurodegenerative diseases, including amyotrophic lateral sclerosis, Parkinson's disease and Huntington's disease. Under conditions of cellular stress, activation of the heat shock response (HSR) results in an increase in the levels of molecular chaperones and is a first line of cellular defence against inclusion formation. It remains to be established whether neurodegenerative disease-associated proteins and inclusions are themselves capable of inducing an HSR in neuronal cells. To address this, we generated a neuroblastoma cell line that expresses a fluorescent reporter protein under conditions of heat shock transcription factor 1-mediated HSR induction. We show that the HSR is not induced by exogenous treatment with aggregated forms of recombinant α-synuclein or the G93A mutant of superoxide dismutase-1 (SOD1G93A) nor intracellular expression of SOD1G93A or a pathogenic form of polyQ-expanded huntingtin (Htt72Q). These results suggest that pathogenic proteins evade detection or impair induction of the HSR in neuronal cells. A failure of protein aggregation to induce an HSR may contribute to the development of inclusion pathology in neurodegenerative diseases.

Publication Date


  • 2020

Citation


  • Gil, R. S., Cox, D., McAlary, L., Berg, T., Walker, A. K., Yerbury, J. J., . . . Ecroyd, H. (2020). Neurodegenerative disease-associated protein aggregates are poor inducers of the heat shock response in neuronal cells.. J Cell Sci. doi:10.1242/jcs.243709

Web Of Science Accession Number