Skip to main content
placeholder image

Effects of naringenin and its phase II metabolites on in vitro human macrophage gene expression

Journal Article


Abstract


  • Naringenin, together with its glycosidic forms, is a flavanone abundant in grapefruit and orange. It has been detected in human plasma, following citrus juice intake, at sub-μmolar concentrations, and its main phase II conjugated metabolites (naringenin-7-O-glucuronide and narigenin-4'-O-glucuronide) have been identified in urine. Recent evidence suggests a potential active anti-inflammatory role of flavonoids on macrophages, cells actively involved in atherogenesis. The aim of this study was to evaluate the effects of naringenin and its phase II metabolites on the expression of specific genes in differently activated macrophages at concentrations coherent with dietary exposure. Results suggest that phase II metabolites, as well as the aglyconic form of naringenin, were able to perturb macrophage gene expression in directions that are not always consistent with anti-inflammatory effects. Moreover, the effects of metabolites were not always consistent with each other and with those of their aglycone, underlining the paramount importance of testing physiological forms of phytochemicals within in vitro experimental models. In vivo studies are needed to further explore these observations and investigate their practical consequences. © 2013 Informa UK Ltd. All rights reserved.

Publication Date


  • 2013

Citation


  • Dall'Asta, M., Derlindati, E., Curella, V., Mena, P., Calani, L., Ray, S., . . . Del Rio, D. (2013). Effects of naringenin and its phase II metabolites on in vitro human macrophage gene expression. International Journal of Food Sciences and Nutrition, 64(7), 843-849. doi:10.3109/09637486.2013.804039

Scopus Eid


  • 2-s2.0-84885391186

Web Of Science Accession Number


Start Page


  • 843

End Page


  • 849

Volume


  • 64

Issue


  • 7

Abstract


  • Naringenin, together with its glycosidic forms, is a flavanone abundant in grapefruit and orange. It has been detected in human plasma, following citrus juice intake, at sub-μmolar concentrations, and its main phase II conjugated metabolites (naringenin-7-O-glucuronide and narigenin-4'-O-glucuronide) have been identified in urine. Recent evidence suggests a potential active anti-inflammatory role of flavonoids on macrophages, cells actively involved in atherogenesis. The aim of this study was to evaluate the effects of naringenin and its phase II metabolites on the expression of specific genes in differently activated macrophages at concentrations coherent with dietary exposure. Results suggest that phase II metabolites, as well as the aglyconic form of naringenin, were able to perturb macrophage gene expression in directions that are not always consistent with anti-inflammatory effects. Moreover, the effects of metabolites were not always consistent with each other and with those of their aglycone, underlining the paramount importance of testing physiological forms of phytochemicals within in vitro experimental models. In vivo studies are needed to further explore these observations and investigate their practical consequences. © 2013 Informa UK Ltd. All rights reserved.

Publication Date


  • 2013

Citation


  • Dall'Asta, M., Derlindati, E., Curella, V., Mena, P., Calani, L., Ray, S., . . . Del Rio, D. (2013). Effects of naringenin and its phase II metabolites on in vitro human macrophage gene expression. International Journal of Food Sciences and Nutrition, 64(7), 843-849. doi:10.3109/09637486.2013.804039

Scopus Eid


  • 2-s2.0-84885391186

Web Of Science Accession Number


Start Page


  • 843

End Page


  • 849

Volume


  • 64

Issue


  • 7