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Sporulation and sterilization method for axenic culture of Gelidium canariensis

Journal Article


Abstract


  • A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2 min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml- 1 each) and 0.1 g ml- 1 of GeO2 in liquid PES for 45 days, during which clusters of spores (85-100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis. �� 1999 Elsevier B.V. All rights reserved.

Publication Date


  • 1999

Citation


  • Garcia-Jimenez, P., Marian, D., Rodrigo, M., & Robaina, R. R. (1999). Sporulation and sterilization method for axenic culture of Gelidium canariensis. Progress in Industrial Microbiology, 35(C), 227-229. doi:10.1016/S0079-6352(99)80117-9

Scopus Eid


  • 2-s2.0-77957806933

Web Of Science Accession Number


Start Page


  • 227

End Page


  • 229

Volume


  • 35

Issue


  • C

Place Of Publication


Abstract


  • A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2 min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml- 1 each) and 0.1 g ml- 1 of GeO2 in liquid PES for 45 days, during which clusters of spores (85-100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis. �� 1999 Elsevier B.V. All rights reserved.

Publication Date


  • 1999

Citation


  • Garcia-Jimenez, P., Marian, D., Rodrigo, M., & Robaina, R. R. (1999). Sporulation and sterilization method for axenic culture of Gelidium canariensis. Progress in Industrial Microbiology, 35(C), 227-229. doi:10.1016/S0079-6352(99)80117-9

Scopus Eid


  • 2-s2.0-77957806933

Web Of Science Accession Number


Start Page


  • 227

End Page


  • 229

Volume


  • 35

Issue


  • C

Place Of Publication