In contrast to the detection and resolution of Late Pleistocene interstadials (Marine Isotope Stages; MIS 5c, 5a and 3) in marine and ice core-based oxygen isotope records, accurately defining palaeosea-levels for these events from sea level indicators remains a challenge. Commonly, such investigations have been undertaken in tectonically active, subduction-related settings, on emergent marginal marine-coastal successions above present sea level, given that the higher ice volumes during these Marine Isotope Stages resulted in ice-equivalent sea levels significantly below present, particularly in far-field settings. Delineation of palaeosea-levels for this period, has accordingly involved an assumption about the long-term rate of coastal uplift. Core SV#23 collected from the tectonically highly stable, remote far-field setting of southern Gulf St Vincent, southern Australia, in a present water depth of 40 m obtained a 3.89 m undisturbed sedimentary record of Late Pleistocene to Holocene age. The basal 2.39 m of the sediment record is of Late Pleistocene interstadial age, as it overlies the Last Interglacial Glanville Formation (128–118 ka; MIS 5e) and occurs beneath the Holocene St Kilda Formation. The extent of aspartic acid and glutamic acid racemization in the benthic foraminifer Elphidium macelliforme sampled at 2 cm intervals continuously from the interstadial succession in Core SV#23, confirms the relative age interpreted from lithostratigraphy. A mean amino acid racemization (AAR) age of 75 ± 13 ka indicates a correlation with MIS 5a, and highlights the utility of E. macelliforme as a species for AAR geochronology. As the foraminifers more closely define the timing of sedimentation, the interstadial succession is regarded as beyond the range of radiocarbon dating (>50 kyr), as revealed in a comparison of radiocarbon ages of fossil molluscs with AAR ages for E. macelliforme from the same depth intervals within the interstadial succession. All of the radiocarbon ages for fossil marine molluscs from the interstadial succession represent minimum ages, resulting from contamination by trace levels of modern radiocarbon. The foraminiferal faunal assemblages within the interstadial succession reveal changes of water depth at the time of deposition, pointing to millennial-scale sea level variability. In particular, the ratio of Elphidium crispum to E. macelliforme indicates several oscillations in relative sea level within a bandwidth of 26 to 24 ± 4.6 m below present sea level (BPSL). Notwithstanding the large uncertainty associated with palaeosea-level estimation based on the Elphidium ratio, the inferred relative sea level record is in accord with the sea level records of Barbados and the Red Sea, and points to Sub-Milankovitch interstadial sea level variability as noted in Greenland and Antarctic ice cores.