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High-resolution and specific detection of bacteria on complex surfaces using nanoparticle probes and electron microscopy.

Journal Article


Abstract


  • The study of the interaction of bacteria with surfaces requires the detection of specific bacterial groups with high spatial resolution. Here, we describe a method to rapidly and efficiently add nanogold particles to oligonucleotide probes, which target bacterial ribosomal RNA. These nanogold-labeled probes are then used in an in situ hybridization procedure that ensures both cellular integrity and high specificity. Electron microscopy subsequently enables the visualization of specific cells with high local precision on complex surface structures. This method will contribute to an increased understanding of how bacteria interact with surface structures on a sub-micron scale.

UOW Authors


  •   Joseph, Stephen (external author)

Publication Date


  • 2015

Citation


  • Ye, J., Nielsen, S., Joseph, S., & Thomas, T. (2015). High-resolution and specific detection of bacteria on complex surfaces using nanoparticle probes and electron microscopy.. PloS one, 10(5), e0126404. doi:10.1371/journal.pone.0126404

Web Of Science Accession Number


Start Page


  • e0126404

Volume


  • 10

Issue


  • 5

Place Of Publication


Abstract


  • The study of the interaction of bacteria with surfaces requires the detection of specific bacterial groups with high spatial resolution. Here, we describe a method to rapidly and efficiently add nanogold particles to oligonucleotide probes, which target bacterial ribosomal RNA. These nanogold-labeled probes are then used in an in situ hybridization procedure that ensures both cellular integrity and high specificity. Electron microscopy subsequently enables the visualization of specific cells with high local precision on complex surface structures. This method will contribute to an increased understanding of how bacteria interact with surface structures on a sub-micron scale.

UOW Authors


  •   Joseph, Stephen (external author)

Publication Date


  • 2015

Citation


  • Ye, J., Nielsen, S., Joseph, S., & Thomas, T. (2015). High-resolution and specific detection of bacteria on complex surfaces using nanoparticle probes and electron microscopy.. PloS one, 10(5), e0126404. doi:10.1371/journal.pone.0126404

Web Of Science Accession Number


Start Page


  • e0126404

Volume


  • 10

Issue


  • 5

Place Of Publication