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Visceral adipose tissue activated macrophage content and inflammatory adipokine secretion is higher in pre-eclampsia than in healthy pregnancy

Journal Article


Abstract


  • Obesity increases pre-eclampsia (PE) risk. Adipose tissue inflammation may contribute to the clinical syndrome of PE. We compared adipose tissue macrophage infiltration and release of pro-inflammatory adipokines in PE and healthy pregnancy. Subcutaneous and visceral adipose tissue biopsies were collected from healthy (n=13) and PE (n=13) mothers. Basal and lipopolysaccharide (LPS) stimulated adipocyte TNF��, IL-6, CCL-2, and CRP release was measured. Adipose tissue cell densities of activated (cfms+) and total (CD68+) macrophages were determined. In PE only, visceral adipose tissue TNF�� release was increased after LPS stimulation (57 [76] versus 81 [97] pg/ml/��g DNA, P=0.030). Basal TNFa release was negatively correlated insulin sensitivity of visceral adipocytes (r =-0.61, P=0.030) in PE. Visceral adipocyte IL-6 release was increased after LPS stimulation in PE only (566 [696] versus 852 [914] pg/ml/��g DNA, P=0.019). Visceral adipocyte CCL-2 basal (67 [61] versus 187 [219] pg/ml/��gDNA, P=0.049) and stimulated (46 [46] versus 224 [271] pg/ml/��g DNA, P=0.003) release was greater than in subcutaneous adipocytes in PE only. In PE, median TNF mRNA expression in visceral adipose tissue was higher than controls (1.94 [1.13-4.14] versus 0.8 [0.00-1.27] TNF/PPIA ratio, P=0.006). In visceral adipose tissue, CSF1R (a marker of activated macrophages) mRNA expression (24.8[11.0] versus 51.0[29.9] CSF1R/PPIA ratio, P=0.011) and activated (cfms+) macrophage count (6.7[2.6] versus 15.2[8.8] % cfms+/adipocyte, P=0.031) were higher in PE than in controls. In conclusion, our study demonstrates dysregulation of inflammatory pathways predominantly in visceral adipose tissue in PE. Inflammation of visceral adipose tissue may mediate many of the adverse metabolic effects associated with PE.

UOW Authors


  •   Freeman, Dilys (external author)

Publication Date


  • 2017

Citation


  • Huda, S. S., Jordan, F., Bray, J., Love, G., Payne, R., Sattar, N., & Freeman, D. J. (2017). Visceral adipose tissue activated macrophage content and inflammatory adipokine secretion is higher in pre-eclampsia than in healthy pregnancy. Clinical Science, 131(13), 1529-1540. doi:10.1042/CS20160832

Scopus Eid


  • 2-s2.0-85021354596

Web Of Science Accession Number


Start Page


  • 1529

End Page


  • 1540

Volume


  • 131

Issue


  • 13

Place Of Publication


Abstract


  • Obesity increases pre-eclampsia (PE) risk. Adipose tissue inflammation may contribute to the clinical syndrome of PE. We compared adipose tissue macrophage infiltration and release of pro-inflammatory adipokines in PE and healthy pregnancy. Subcutaneous and visceral adipose tissue biopsies were collected from healthy (n=13) and PE (n=13) mothers. Basal and lipopolysaccharide (LPS) stimulated adipocyte TNF��, IL-6, CCL-2, and CRP release was measured. Adipose tissue cell densities of activated (cfms+) and total (CD68+) macrophages were determined. In PE only, visceral adipose tissue TNF�� release was increased after LPS stimulation (57 [76] versus 81 [97] pg/ml/��g DNA, P=0.030). Basal TNFa release was negatively correlated insulin sensitivity of visceral adipocytes (r =-0.61, P=0.030) in PE. Visceral adipocyte IL-6 release was increased after LPS stimulation in PE only (566 [696] versus 852 [914] pg/ml/��g DNA, P=0.019). Visceral adipocyte CCL-2 basal (67 [61] versus 187 [219] pg/ml/��gDNA, P=0.049) and stimulated (46 [46] versus 224 [271] pg/ml/��g DNA, P=0.003) release was greater than in subcutaneous adipocytes in PE only. In PE, median TNF mRNA expression in visceral adipose tissue was higher than controls (1.94 [1.13-4.14] versus 0.8 [0.00-1.27] TNF/PPIA ratio, P=0.006). In visceral adipose tissue, CSF1R (a marker of activated macrophages) mRNA expression (24.8[11.0] versus 51.0[29.9] CSF1R/PPIA ratio, P=0.011) and activated (cfms+) macrophage count (6.7[2.6] versus 15.2[8.8] % cfms+/adipocyte, P=0.031) were higher in PE than in controls. In conclusion, our study demonstrates dysregulation of inflammatory pathways predominantly in visceral adipose tissue in PE. Inflammation of visceral adipose tissue may mediate many of the adverse metabolic effects associated with PE.

UOW Authors


  •   Freeman, Dilys (external author)

Publication Date


  • 2017

Citation


  • Huda, S. S., Jordan, F., Bray, J., Love, G., Payne, R., Sattar, N., & Freeman, D. J. (2017). Visceral adipose tissue activated macrophage content and inflammatory adipokine secretion is higher in pre-eclampsia than in healthy pregnancy. Clinical Science, 131(13), 1529-1540. doi:10.1042/CS20160832

Scopus Eid


  • 2-s2.0-85021354596

Web Of Science Accession Number


Start Page


  • 1529

End Page


  • 1540

Volume


  • 131

Issue


  • 13

Place Of Publication