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Full integration and automation of immunoassay protocols by rotationally actuated dissolvable film valves

Conference Paper


Abstract


  • Here we describe a fully integrated and automated, multi-step immunoassay protocol utilizing our water-dissolvable films (DFs) actuated, centrifugo-pneumatic valving scheme [1]. This technique establishes a sequence of laboratory unit operations comprising of blood separation, metering, mixing/incubation, and liquid reagent release from on-board reservoirs for washing and detection by mere control of the rotational frequency. During storage at rest and at low rotational frequencies, the sacrificial DF valves provide permanent liquid and vapor barriers. However, beyond a critical burst frequency ranging up to 3500 rotations per minute (rpm), these valves yield by inverting a metastable gas-liquid layer to trigger the wetting and thus dissolution of the DFs.

Publication Date


  • 2011

Citation


  • Nwankire, C., Gorkin, R., Siegrist, J., Gaughran, J., Chan, D. S., & Ducrée, J. (2011). Full integration and automation of immunoassay protocols by rotationally actuated dissolvable film valves. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 Vol. 3 (pp. 1665-1667).

Scopus Eid


  • 2-s2.0-84883755443

Web Of Science Accession Number


Start Page


  • 1665

End Page


  • 1667

Volume


  • 3

Abstract


  • Here we describe a fully integrated and automated, multi-step immunoassay protocol utilizing our water-dissolvable films (DFs) actuated, centrifugo-pneumatic valving scheme [1]. This technique establishes a sequence of laboratory unit operations comprising of blood separation, metering, mixing/incubation, and liquid reagent release from on-board reservoirs for washing and detection by mere control of the rotational frequency. During storage at rest and at low rotational frequencies, the sacrificial DF valves provide permanent liquid and vapor barriers. However, beyond a critical burst frequency ranging up to 3500 rotations per minute (rpm), these valves yield by inverting a metastable gas-liquid layer to trigger the wetting and thus dissolution of the DFs.

Publication Date


  • 2011

Citation


  • Nwankire, C., Gorkin, R., Siegrist, J., Gaughran, J., Chan, D. S., & Ducrée, J. (2011). Full integration and automation of immunoassay protocols by rotationally actuated dissolvable film valves. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 Vol. 3 (pp. 1665-1667).

Scopus Eid


  • 2-s2.0-84883755443

Web Of Science Accession Number


Start Page


  • 1665

End Page


  • 1667

Volume


  • 3