Skip to main content
placeholder image

Enhanced microfabrication capabilities of thermoplastics elastomers for CD lab system including: Lysing, PCR and hybridization microfluidic functions

Conference Paper


Abstract


  • We demonstrate the feasibility to fabricate and assemble at low-cost a complex multi-level microfluidic CD hybrid system made through the assembly of only two polymer layers. The design demonstrated in this work integrate in a single molded thermoplastic elastomer (TPE) layer all the microfluidic features necessary to build a nucleic acid testing device. This design would allow for cell lysis, sample metering and clarification, mixing with PCR buffer, PCR amplification, exonuclease digestion of amplified DNA, self-venting and finally DNA hybridization on a microarray located on the upper layer of the CD.

Publication Date


  • 2010

Citation


  • Roy, E., Mounier, M., Peytavi, R., Siegrist, J., Gorkin, R., Madou, M., . . . Veres, T. (2010). Enhanced microfabrication capabilities of thermoplastics elastomers for CD lab system including: Lysing, PCR and hybridization microfluidic functions. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 Vol. 3 (pp. 1898-1899).

Scopus Eid


  • 2-s2.0-84855946388

Web Of Science Accession Number


Start Page


  • 1898

End Page


  • 1899

Volume


  • 3

Abstract


  • We demonstrate the feasibility to fabricate and assemble at low-cost a complex multi-level microfluidic CD hybrid system made through the assembly of only two polymer layers. The design demonstrated in this work integrate in a single molded thermoplastic elastomer (TPE) layer all the microfluidic features necessary to build a nucleic acid testing device. This design would allow for cell lysis, sample metering and clarification, mixing with PCR buffer, PCR amplification, exonuclease digestion of amplified DNA, self-venting and finally DNA hybridization on a microarray located on the upper layer of the CD.

Publication Date


  • 2010

Citation


  • Roy, E., Mounier, M., Peytavi, R., Siegrist, J., Gorkin, R., Madou, M., . . . Veres, T. (2010). Enhanced microfabrication capabilities of thermoplastics elastomers for CD lab system including: Lysing, PCR and hybridization microfluidic functions. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 Vol. 3 (pp. 1898-1899).

Scopus Eid


  • 2-s2.0-84855946388

Web Of Science Accession Number


Start Page


  • 1898

End Page


  • 1899

Volume


  • 3