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Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer

Conference Paper


Abstract


  • In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent® (TRI) to provide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase separated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent resistant cyclo olefin polymer (Zeonor®) disc was used in this case.

Publication Date


  • 2011

Citation


  • Linares, A. V., Gorkin, R., Glynn, B., Godino, N., Miller, N., Kerin, M., . . . Ducrée, J. (2011). Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 Vol. 3 (pp. 1460-1462).

Scopus Eid


  • 2-s2.0-84883745600

Web Of Science Accession Number


Start Page


  • 1460

End Page


  • 1462

Volume


  • 3

Abstract


  • In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent® (TRI) to provide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase separated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent resistant cyclo olefin polymer (Zeonor®) disc was used in this case.

Publication Date


  • 2011

Citation


  • Linares, A. V., Gorkin, R., Glynn, B., Godino, N., Miller, N., Kerin, M., . . . Ducrée, J. (2011). Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 Vol. 3 (pp. 1460-1462).

Scopus Eid


  • 2-s2.0-84883745600

Web Of Science Accession Number


Start Page


  • 1460

End Page


  • 1462

Volume


  • 3