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Efficient development kit for well-to-chip customization and detection of colorimetric and fluorescence based microfluidic immunoassays

Conference Paper


Abstract


  • Microfluidics is advantageous for immunoassay processing (e.g., reducing immunoreagent consumption/assay times) [1], but the fact that most clinically relevant bioassays are developed for well-plate technologies poses significant challenges for integration within μTAS platforms. Assays developed for 96-well plates must be downscaled and adapted for microfluidic settings, involving difficulties in volume and surface chemistry optimization in addition to the inability for microfluidic detection using conventional analytical approaches. To address the need for performing replicate immunoassays in microfluidic environments, we report on the development of translational, slide-based microfluidic cartridges integrated with specialized detection technologies (fluorescence and colorimetric) that enable miniaturization/ analysis of standard assays. Copyright © (2011) by the Chemical and Biological Microsystems Society.

Publication Date


  • 2011

Citation


  • Gorkin, R., Burger, R., Kurzbuch, D., Donohoe, G. G., Zhang, X., Czugala, M., . . . Ducrée, J. (2011). Efficient development kit for well-to-chip customization and detection of colorimetric and fluorescence based microfluidic immunoassays. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 Vol. 2 (pp. 924-926).

Scopus Eid


  • 2-s2.0-84883785207

Web Of Science Accession Number


Start Page


  • 924

End Page


  • 926

Volume


  • 2

Abstract


  • Microfluidics is advantageous for immunoassay processing (e.g., reducing immunoreagent consumption/assay times) [1], but the fact that most clinically relevant bioassays are developed for well-plate technologies poses significant challenges for integration within μTAS platforms. Assays developed for 96-well plates must be downscaled and adapted for microfluidic settings, involving difficulties in volume and surface chemistry optimization in addition to the inability for microfluidic detection using conventional analytical approaches. To address the need for performing replicate immunoassays in microfluidic environments, we report on the development of translational, slide-based microfluidic cartridges integrated with specialized detection technologies (fluorescence and colorimetric) that enable miniaturization/ analysis of standard assays. Copyright © (2011) by the Chemical and Biological Microsystems Society.

Publication Date


  • 2011

Citation


  • Gorkin, R., Burger, R., Kurzbuch, D., Donohoe, G. G., Zhang, X., Czugala, M., . . . Ducrée, J. (2011). Efficient development kit for well-to-chip customization and detection of colorimetric and fluorescence based microfluidic immunoassays. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 Vol. 2 (pp. 924-926).

Scopus Eid


  • 2-s2.0-84883785207

Web Of Science Accession Number


Start Page


  • 924

End Page


  • 926

Volume


  • 2