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An acetazolamide-sensitive inward chloride pump in vascular smooth muscle

Journal Article


Abstract


  • In smooth muscle cells, which have a small but significant chloride ion permeability, Cl:HCO3 exchange and (Na+K+Cl) cotransport are known to act as inwardly directed chloride pumps. However, even allowing for overestimation of [Cl](i) due to intracellular interference with the Cl-recording electrodes, there remained a residual accumulation of chloride in rat arterial smooth muscle under conditions in which neither of these processes is operative. Manipulation of [Cl](i) and Cl permeability using pharmacological agents whilst monitoring both E(m) and [Cl](i) showed that an acetazolamide-sensitive mechanism, perhaps a Cl-dependent ATPase, is the third pathway for Cl accumulation. © 1993 Academic Press, Inc.

UOW Authors


  •   Harper, Alexander (external author)

Publication Date


  • 1993

Citation


  • Chipperfield, A. R., Davis, J. P. L., & Harper, A. A. (1993). An acetazolamide-sensitive inward chloride pump in vascular smooth muscle. Biochemical and Biophysical Research Communications, 194(1), 407-412. doi:10.1006/bbrc.1993.1834

Scopus Eid


  • 2-s2.0-0027237015

Start Page


  • 407

End Page


  • 412

Volume


  • 194

Issue


  • 1

Abstract


  • In smooth muscle cells, which have a small but significant chloride ion permeability, Cl:HCO3 exchange and (Na+K+Cl) cotransport are known to act as inwardly directed chloride pumps. However, even allowing for overestimation of [Cl](i) due to intracellular interference with the Cl-recording electrodes, there remained a residual accumulation of chloride in rat arterial smooth muscle under conditions in which neither of these processes is operative. Manipulation of [Cl](i) and Cl permeability using pharmacological agents whilst monitoring both E(m) and [Cl](i) showed that an acetazolamide-sensitive mechanism, perhaps a Cl-dependent ATPase, is the third pathway for Cl accumulation. © 1993 Academic Press, Inc.

UOW Authors


  •   Harper, Alexander (external author)

Publication Date


  • 1993

Citation


  • Chipperfield, A. R., Davis, J. P. L., & Harper, A. A. (1993). An acetazolamide-sensitive inward chloride pump in vascular smooth muscle. Biochemical and Biophysical Research Communications, 194(1), 407-412. doi:10.1006/bbrc.1993.1834

Scopus Eid


  • 2-s2.0-0027237015

Start Page


  • 407

End Page


  • 412

Volume


  • 194

Issue


  • 1