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The gene associated with trichorhinophalangeal syndrome in humans is overexpressed in breast cancer.

Journal Article


Abstract


  • A comprehensive differential gene expression screen on a panel of 54 breast tumors and >200 normal tissue samples using DNA microarrays revealed 15 genes specifically overexpressed in breast cancer. One of the most prevalent genes found was trichorhinophalangeal syndrome type 1 (TRPS-1), a gene previously shown to be associated with three rare autosomal dominant genetic disorders known as the trichorhinophalangeal syndromes. A number of corroborating methodologies, including in situ hybridization, e-Northern analysis using ORF EST (ORESTES) and Unigene EST abundance analysis, immunoblot and immunofluorescence analysis of breast tumor cell lines, and immunohistochemistry, confirmed the microarray findings. Immunohistochemistry analysis found TRPS-1 protein expressed in >90% of early- and late-stage breast cancer, including ductal carcinoma in situ and invasive ductal, lobular, and papillary carcinomas. The TRPS-1 gene is also immunogenic with processed and presented peptides activating T cells found after vaccination of HLA-A2.1 transgenic mouse. Human T cell lines from HLA-A*0201+ female donors exhibiting TRPS-1-specific cytotoxic T lymphocyte activity could also be generated.

UOW Authors


  •   Venter, Deon (external author)

Publication Date


  • 2005

Citation


  • Radvanyi, L., Singh-Sandhu, D., Gallichan, S., Lovitt, C., Pedyczak, A., Mallo, G., . . . Berinstein, N. L. (2005). The gene associated with trichorhinophalangeal syndrome in humans is overexpressed in breast cancer.. Proceedings of the National Academy of Sciences of the United States of America, 102(31), 11005-11010. doi:10.1073/pnas.0500904102

Web Of Science Accession Number


Start Page


  • 11005

End Page


  • 11010

Volume


  • 102

Issue


  • 31

Abstract


  • A comprehensive differential gene expression screen on a panel of 54 breast tumors and >200 normal tissue samples using DNA microarrays revealed 15 genes specifically overexpressed in breast cancer. One of the most prevalent genes found was trichorhinophalangeal syndrome type 1 (TRPS-1), a gene previously shown to be associated with three rare autosomal dominant genetic disorders known as the trichorhinophalangeal syndromes. A number of corroborating methodologies, including in situ hybridization, e-Northern analysis using ORF EST (ORESTES) and Unigene EST abundance analysis, immunoblot and immunofluorescence analysis of breast tumor cell lines, and immunohistochemistry, confirmed the microarray findings. Immunohistochemistry analysis found TRPS-1 protein expressed in >90% of early- and late-stage breast cancer, including ductal carcinoma in situ and invasive ductal, lobular, and papillary carcinomas. The TRPS-1 gene is also immunogenic with processed and presented peptides activating T cells found after vaccination of HLA-A2.1 transgenic mouse. Human T cell lines from HLA-A*0201+ female donors exhibiting TRPS-1-specific cytotoxic T lymphocyte activity could also be generated.

UOW Authors


  •   Venter, Deon (external author)

Publication Date


  • 2005

Citation


  • Radvanyi, L., Singh-Sandhu, D., Gallichan, S., Lovitt, C., Pedyczak, A., Mallo, G., . . . Berinstein, N. L. (2005). The gene associated with trichorhinophalangeal syndrome in humans is overexpressed in breast cancer.. Proceedings of the National Academy of Sciences of the United States of America, 102(31), 11005-11010. doi:10.1073/pnas.0500904102

Web Of Science Accession Number


Start Page


  • 11005

End Page


  • 11010

Volume


  • 102

Issue


  • 31