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DOTAP functionalizing single-walled carbon nanotubes as non-viral vectors for efficient intracellular siRNA delivery.

Journal Article


Abstract


  • Context

    Functionalized single-walled carbon nanotubes (SWNT) with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used as novel and more convenient carriers of small interfering RNA (siRNA).

    Objective

    To utilize the unique capability of SWNT to be easily modified by functional groups and readily internalized by mammalian cells to bind, condense, stabilize siRNA and enhance its transfection efficiency.

    Methods

    After SWNT were non-covalently functionalized by cationic DOTAP (SWNT-DOTAP), siRNA interacted with SWNT-DOTAP via static electricity (SWNT-DOTAP/siRNA). Subsequently, the size, zeta potential and morphology of SWNT-DOTAP/siRNA were analyzed. The optimal compression ratio and stability of siRNA were assessed by agarose gel electrophoresis. Furthermore, in prostate carcinoma PC-3 cells, RT-PCR, flow cytometry and sulforhodamine B assays were used to evaluate the silencing activity, transfection efficiency and cell proliferation, respectively.

    Results and discussion

    The characteristics of SWNT-DOTAP, i.e. an average size of 194.49 nm, a zeta potential of 45.16 mV and lower cytotoxicity than Lipofectamine 2000, indicated that this vector was suitable for siRNA delivery. Moreover, after interaction with SWNT-DOTAP, siRNA of human telomerase reverse transcriptase was bound, condensed and stabilized. In PC-3 cells, SWNT-DOTAP/siRNA exhibited 82.6% silencing activity and 92% transfection efficiency. Furthermore, the complexes inhibited cell proliferation by 42.1%.

    Conclusion

    SWNT-DOTAP may be a promising siRNA delivery vector for gene-based therapeutic applications in cancer.

Publication Date


  • 2014

Citation


  • Li, H., Hao, Y., Wang, N., Wang, L., Jia, S., Wang, Y., . . . Zhang, Z. (2016). DOTAP functionalizing single-walled carbon nanotubes as non-viral vectors for efficient intracellular siRNA delivery.. Drug delivery, 23(3), 840-848. doi:10.3109/10717544.2014.919542

Web Of Science Accession Number


Start Page


  • 840

End Page


  • 848

Volume


  • 23

Issue


  • 3

Abstract


  • Context

    Functionalized single-walled carbon nanotubes (SWNT) with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used as novel and more convenient carriers of small interfering RNA (siRNA).

    Objective

    To utilize the unique capability of SWNT to be easily modified by functional groups and readily internalized by mammalian cells to bind, condense, stabilize siRNA and enhance its transfection efficiency.

    Methods

    After SWNT were non-covalently functionalized by cationic DOTAP (SWNT-DOTAP), siRNA interacted with SWNT-DOTAP via static electricity (SWNT-DOTAP/siRNA). Subsequently, the size, zeta potential and morphology of SWNT-DOTAP/siRNA were analyzed. The optimal compression ratio and stability of siRNA were assessed by agarose gel electrophoresis. Furthermore, in prostate carcinoma PC-3 cells, RT-PCR, flow cytometry and sulforhodamine B assays were used to evaluate the silencing activity, transfection efficiency and cell proliferation, respectively.

    Results and discussion

    The characteristics of SWNT-DOTAP, i.e. an average size of 194.49 nm, a zeta potential of 45.16 mV and lower cytotoxicity than Lipofectamine 2000, indicated that this vector was suitable for siRNA delivery. Moreover, after interaction with SWNT-DOTAP, siRNA of human telomerase reverse transcriptase was bound, condensed and stabilized. In PC-3 cells, SWNT-DOTAP/siRNA exhibited 82.6% silencing activity and 92% transfection efficiency. Furthermore, the complexes inhibited cell proliferation by 42.1%.

    Conclusion

    SWNT-DOTAP may be a promising siRNA delivery vector for gene-based therapeutic applications in cancer.

Publication Date


  • 2014

Citation


  • Li, H., Hao, Y., Wang, N., Wang, L., Jia, S., Wang, Y., . . . Zhang, Z. (2016). DOTAP functionalizing single-walled carbon nanotubes as non-viral vectors for efficient intracellular siRNA delivery.. Drug delivery, 23(3), 840-848. doi:10.3109/10717544.2014.919542

Web Of Science Accession Number


Start Page


  • 840

End Page


  • 848

Volume


  • 23

Issue


  • 3