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Structure of a calpain Ca2+-binding domain reveals a novel EF-hand and Ca2+-induced conformational changes

Journal Article


Abstract


  • The crystal structure of a Ca2+-binding domain (dVI) of rat m-calpain has been determined at 2.3 Å resolution, both with and without bound Ca2+. The structures reveal a unique fold incorporating five EF-hand motifs per monomer, three of which bind calcium at physiological calcium concentrations, with one showing a novel EF-hand coordination pattern. This investigation gives us a first view of the calcium-induced conformational changes, and consequently an insight into the mechanism of calcium induced activation in calpain. The crystal structures reveal a dVI homodimer which provides a preliminary model for the subunit dimerization in calpain.

UOW Authors


  •   Blanchard, Helen (external author)

Publication Date


  • 1997

Citation


  • Blanchard, H., Grochulski, P., Li, Y., Arthur, J. S. C., Davies, P. L., Elce, J. S., & Cygler, M. (1997). Structure of a calpain Ca2+-binding domain reveals a novel EF-hand and Ca2+-induced conformational changes. Nature Structural Biology, 4(7), 532-538. doi:10.1038/nsb0797-532

Scopus Eid


  • 2-s2.0-0030998285

Start Page


  • 532

End Page


  • 538

Volume


  • 4

Issue


  • 7

Abstract


  • The crystal structure of a Ca2+-binding domain (dVI) of rat m-calpain has been determined at 2.3 Å resolution, both with and without bound Ca2+. The structures reveal a unique fold incorporating five EF-hand motifs per monomer, three of which bind calcium at physiological calcium concentrations, with one showing a novel EF-hand coordination pattern. This investigation gives us a first view of the calcium-induced conformational changes, and consequently an insight into the mechanism of calcium induced activation in calpain. The crystal structures reveal a dVI homodimer which provides a preliminary model for the subunit dimerization in calpain.

UOW Authors


  •   Blanchard, Helen (external author)

Publication Date


  • 1997

Citation


  • Blanchard, H., Grochulski, P., Li, Y., Arthur, J. S. C., Davies, P. L., Elce, J. S., & Cygler, M. (1997). Structure of a calpain Ca2+-binding domain reveals a novel EF-hand and Ca2+-induced conformational changes. Nature Structural Biology, 4(7), 532-538. doi:10.1038/nsb0797-532

Scopus Eid


  • 2-s2.0-0030998285

Start Page


  • 532

End Page


  • 538

Volume


  • 4

Issue


  • 7