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Sialic acid dependence in rotavirus host cell invasion

Journal Article


Abstract


  • We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8*) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides GM1 and GD1a. Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses. © 2009 Nature America, Inc. All rights reserved.

UOW Authors


  •   Blanchard, Helen (external author)

Publication Date


  • 2009

Citation


  • Haselhorst, T., Fleming, F. E., Dyason, J. C., Hartnell, R. D., Yu, X., Holloway, G., . . . Von Itzstein, M. (2009). Sialic acid dependence in rotavirus host cell invasion. Nature Chemical Biology, 5(2), 91-93. doi:10.1038/nchembio.134

Scopus Eid


  • 2-s2.0-58249118009

Start Page


  • 91

End Page


  • 93

Volume


  • 5

Issue


  • 2

Abstract


  • We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8*) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides GM1 and GD1a. Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses. © 2009 Nature America, Inc. All rights reserved.

UOW Authors


  •   Blanchard, Helen (external author)

Publication Date


  • 2009

Citation


  • Haselhorst, T., Fleming, F. E., Dyason, J. C., Hartnell, R. D., Yu, X., Holloway, G., . . . Von Itzstein, M. (2009). Sialic acid dependence in rotavirus host cell invasion. Nature Chemical Biology, 5(2), 91-93. doi:10.1038/nchembio.134

Scopus Eid


  • 2-s2.0-58249118009

Start Page


  • 91

End Page


  • 93

Volume


  • 5

Issue


  • 2