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Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CRW-8

Journal Article


Abstract


  • Rotavirus recognition and attachment to host cells involves interaction with the spike protein VP4 that projects outwards from the surface of the virus particle. An integral component of these spikes is the VP8* domain, which is implicated in the direct recognition and binding of sialic acid-containing cell-surface carbohydrates and facilitates subsequent invasion by the virus. The expression, purification, crystallization and preliminary X-ray diffraction analysis of VP8* from porcine CRW-8 rotavirus is reported. Diffraction data have been collected to 2.3 Å resolution, enabling the determination of the VP8* structure by molecular replacement. © 2005 International Union of Crystallography. All rights reserved.

Publication Date


  • 2005

Citation


  • Scott, S. A., Holloway, G., Coulson, B. S., Szyczew, A. J., Kiefel, M. J., Von Itzstein, M., & Blanchard, H. (2005). Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CRW-8. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(6), 617-620. doi:10.1107/S1744309105013849

Scopus Eid


  • 2-s2.0-33744465800

Start Page


  • 617

End Page


  • 620

Volume


  • 61

Issue


  • 6

Abstract


  • Rotavirus recognition and attachment to host cells involves interaction with the spike protein VP4 that projects outwards from the surface of the virus particle. An integral component of these spikes is the VP8* domain, which is implicated in the direct recognition and binding of sialic acid-containing cell-surface carbohydrates and facilitates subsequent invasion by the virus. The expression, purification, crystallization and preliminary X-ray diffraction analysis of VP8* from porcine CRW-8 rotavirus is reported. Diffraction data have been collected to 2.3 Å resolution, enabling the determination of the VP8* structure by molecular replacement. © 2005 International Union of Crystallography. All rights reserved.

Publication Date


  • 2005

Citation


  • Scott, S. A., Holloway, G., Coulson, B. S., Szyczew, A. J., Kiefel, M. J., Von Itzstein, M., & Blanchard, H. (2005). Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CRW-8. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(6), 617-620. doi:10.1107/S1744309105013849

Scopus Eid


  • 2-s2.0-33744465800

Start Page


  • 617

End Page


  • 620

Volume


  • 61

Issue


  • 6