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Visualizing viral fusion at the single-particle level in microchannels

Conference Paper


Abstract


  • Membrane fusion is an essential step during entry of enveloped viruses into cells. Conventional fusion assays are generally limited to observation of ensembles of multiple fusion events, confounding more detailed analysis of the sequence of the molecular steps involved. We have developed ultra-sensitive fluorescence imaging techniques that allow the visualization of membrane fusion by a single viral particle. The combination of such single-molecule imaging methods with the use of microfluidics enable the highly multiplexed observation of viral fusion events, facilitating both mechanistic analyses and the characterization of fusion-inhibiting drug candidates.

Publication Date


  • 2010

Citation


  • Floyd, D. L., Otterstrom, J. J., Skehel, J. J., Harrison, S. C., & Van Oijen, A. M. (2010). Visualizing viral fusion at the single-particle level in microchannels. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 Vol. 2 (pp. 1412-1414).

Scopus Eid


  • 2-s2.0-84884395087

Web Of Science Accession Number


Start Page


  • 1412

End Page


  • 1414

Volume


  • 2

Abstract


  • Membrane fusion is an essential step during entry of enveloped viruses into cells. Conventional fusion assays are generally limited to observation of ensembles of multiple fusion events, confounding more detailed analysis of the sequence of the molecular steps involved. We have developed ultra-sensitive fluorescence imaging techniques that allow the visualization of membrane fusion by a single viral particle. The combination of such single-molecule imaging methods with the use of microfluidics enable the highly multiplexed observation of viral fusion events, facilitating both mechanistic analyses and the characterization of fusion-inhibiting drug candidates.

Publication Date


  • 2010

Citation


  • Floyd, D. L., Otterstrom, J. J., Skehel, J. J., Harrison, S. C., & Van Oijen, A. M. (2010). Visualizing viral fusion at the single-particle level in microchannels. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 Vol. 2 (pp. 1412-1414).

Scopus Eid


  • 2-s2.0-84884395087

Web Of Science Accession Number


Start Page


  • 1412

End Page


  • 1414

Volume


  • 2