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A hydrodynamic microchip for formation of continuous cell chains

Journal Article


Abstract


  • Here, we demonstrate the unique features of a hydrodynamic based microchip for creating continuous chains of model yeast cells. The system consists of a disk shaped microfluidic structure, containing narrow orifices that connect the main channel to an array of spoke channels. Negative pressure provided by a syringe pump draws fluid from the main channel through the narrow orifices. After cleaning process, a thin layer of water is left between the glass substrate and the polydimethylsiloxane microchip, enabling leakage beneath the channel walls. A mechanical clamp is used to adjust the operation of the microchip. Relaxing the clamp allows leakage of liquid beneath the walls in a controllable fashion, leading to formation of a long cell chain evenly distributed along the channel wall. The unique features of the microchip are demonstrated by creating long chains of yeast cells and model 15��m polystyrene particles along the side wall and analysing the hydrogen peroxide induced death of patterned cells. �� 2014 AIP Publishing LLC.

UOW Authors


  •   Tang, Shiyang (external author)

Publication Date


  • 2014

Citation


  • Khoshmanesh, K., Zhang, W., Tang, S. Y., Nasabi, M., Soffe, R., Tovar-Lopez, F. J., . . . Mitchell, A. (2014). A hydrodynamic microchip for formation of continuous cell chains. Applied Physics Letters, 104(20). doi:10.1063/1.4879457

Scopus Eid


  • 2-s2.0-84901424947

Volume


  • 104

Issue


  • 20

Place Of Publication


Abstract


  • Here, we demonstrate the unique features of a hydrodynamic based microchip for creating continuous chains of model yeast cells. The system consists of a disk shaped microfluidic structure, containing narrow orifices that connect the main channel to an array of spoke channels. Negative pressure provided by a syringe pump draws fluid from the main channel through the narrow orifices. After cleaning process, a thin layer of water is left between the glass substrate and the polydimethylsiloxane microchip, enabling leakage beneath the channel walls. A mechanical clamp is used to adjust the operation of the microchip. Relaxing the clamp allows leakage of liquid beneath the walls in a controllable fashion, leading to formation of a long cell chain evenly distributed along the channel wall. The unique features of the microchip are demonstrated by creating long chains of yeast cells and model 15��m polystyrene particles along the side wall and analysing the hydrogen peroxide induced death of patterned cells. �� 2014 AIP Publishing LLC.

UOW Authors


  •   Tang, Shiyang (external author)

Publication Date


  • 2014

Citation


  • Khoshmanesh, K., Zhang, W., Tang, S. Y., Nasabi, M., Soffe, R., Tovar-Lopez, F. J., . . . Mitchell, A. (2014). A hydrodynamic microchip for formation of continuous cell chains. Applied Physics Letters, 104(20). doi:10.1063/1.4879457

Scopus Eid


  • 2-s2.0-84901424947

Volume


  • 104

Issue


  • 20

Place Of Publication