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Kinetic analysis of enzymatic oleic acid esterification under monophaslc and biphaslc condition

Conference Paper


Abstract


  • The esterification kinetics of oleic acid (OA) using ethanol (EtOH) over macroporous acrylic-supported lipase from Aspergillus niger (Novozym 435) as catalyst was studied. The rate dependency on reactant concentration had a maximum at 0.9 for monophasic reaction, and was described by a pseudo-Michaelis-Menten kinetic expression implicating surface reaction between complex-transformed intermediate and EtOH as the rate-controlling step. Initial water addition amplified the effect of ethanol inhibition and increased 11.4 times of reaction velocity, thus shifting the maximum up to 1.4 in the biphasic reaction in sigmoidal manner. This behavior was of autocatalytic symptom suggesting secondary activation occurred on the lipase surface and was fitly modeled according to Ping Pong Bi Bi mechanism. This is an abstract of a paper presented at the 8th World Congress of Chemical Engineering (Montréal, Quebec, Canada 8/23-27/2009).

Publication Date


  • 2009

Citation


  • Mahmud, M., Safinski, T., Adesina, A., Nelson, M. I., & Sidhu, H. S. (2009). Kinetic analysis of enzymatic oleic acid esterification under monophaslc and biphaslc condition. In 8th World Congress of Chemical Engineering: Incorporating the 59th Canadian Chemical Engineering Conference and the 24th Interamerican Congress of Chemical Engineering.

Scopus Eid


  • 2-s2.0-77951572363

Web Of Science Accession Number


Abstract


  • The esterification kinetics of oleic acid (OA) using ethanol (EtOH) over macroporous acrylic-supported lipase from Aspergillus niger (Novozym 435) as catalyst was studied. The rate dependency on reactant concentration had a maximum at 0.9 for monophasic reaction, and was described by a pseudo-Michaelis-Menten kinetic expression implicating surface reaction between complex-transformed intermediate and EtOH as the rate-controlling step. Initial water addition amplified the effect of ethanol inhibition and increased 11.4 times of reaction velocity, thus shifting the maximum up to 1.4 in the biphasic reaction in sigmoidal manner. This behavior was of autocatalytic symptom suggesting secondary activation occurred on the lipase surface and was fitly modeled according to Ping Pong Bi Bi mechanism. This is an abstract of a paper presented at the 8th World Congress of Chemical Engineering (Montréal, Quebec, Canada 8/23-27/2009).

Publication Date


  • 2009

Citation


  • Mahmud, M., Safinski, T., Adesina, A., Nelson, M. I., & Sidhu, H. S. (2009). Kinetic analysis of enzymatic oleic acid esterification under monophaslc and biphaslc condition. In 8th World Congress of Chemical Engineering: Incorporating the 59th Canadian Chemical Engineering Conference and the 24th Interamerican Congress of Chemical Engineering.

Scopus Eid


  • 2-s2.0-77951572363

Web Of Science Accession Number