Aim: To study the role and possible mechanism of He-Ne laser acupoint irradiation treatment on hypoxia ischemia brain damage (HIBD). Methods: Forty-two Wistar rats, 7 days of age, were randomized into sham-operation group, HIBD group and He-Ne laser acupoint irradiation group (laser group). Twenty-two days later, left brains were taken out and made into paraffin sections. Immunohistochemical staining was performed with choline acetyltransferase (ChAT) and brain-derived neurotrophic factor(BDNF). Results: He-Ne laser acupoint irradiation was effective in inhibiting the loss of Nissl bodies in brain tissue and promoting the expression of ChAT and BDNF in brain tissue after hypoxia-ischemia. Conclusion: He-Ne laser acupoint irradiation has a neuroprotective effect on brain after hypoxia-ischemia, and its action mechanism may be related to the expression of BDNF.