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Studies on immunoprotection of monoclonal antibodies against Cryptosporidium parvum

Journal Article


Abstract

  • AIM: To explore the immune protection of the monoclonal antibody (McAb) against Cryptosporidium parvum. METHODS: On the basis of Madin-Darby canine kidney (MDCK) cell culture, the immunoprotection of McAb against C. parvum was screened by means of neutralization test in vitro, and verified by rat model and transmission electron microscopy of infected MDCK cells. RESULTS: The number of C. parvum sporozoites recovered on the surface of rat intestinal epithelial cells and the mean output of oocyst were significantly reduced by McAb Z3D2 (P < 0.001, P < 0.05). The decreased Cryptos poridium at each development phase within infected MDCK cells and less damage of cell ultrastructure caused by Cryptosporidium were found in Z3D2 treated group. CONCLUSION: McAb Z3D2 possesses highly effective protection against C. parvum.

Publication Date

  • 1999

Citation

  • Zong, H., Li, W., Zhang, X., Yu, S., & Bai, B. (1999). Studies on immunoprotection of monoclonal antibodies against Cryptosporidium parvum. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 17(2), 101-105.

Scopus Eid

  • 2-s2.0-0038824016

Web Of Science Accession Number

Start Page

  • 101

End Page

  • 105

Volume

  • 17

Issue

  • 2

Abstract

  • AIM: To explore the immune protection of the monoclonal antibody (McAb) against Cryptosporidium parvum. METHODS: On the basis of Madin-Darby canine kidney (MDCK) cell culture, the immunoprotection of McAb against C. parvum was screened by means of neutralization test in vitro, and verified by rat model and transmission electron microscopy of infected MDCK cells. RESULTS: The number of C. parvum sporozoites recovered on the surface of rat intestinal epithelial cells and the mean output of oocyst were significantly reduced by McAb Z3D2 (P < 0.001, P < 0.05). The decreased Cryptos poridium at each development phase within infected MDCK cells and less damage of cell ultrastructure caused by Cryptosporidium were found in Z3D2 treated group. CONCLUSION: McAb Z3D2 possesses highly effective protection against C. parvum.

Publication Date

  • 1999

Citation

  • Zong, H., Li, W., Zhang, X., Yu, S., & Bai, B. (1999). Studies on immunoprotection of monoclonal antibodies against Cryptosporidium parvum. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 17(2), 101-105.

Scopus Eid

  • 2-s2.0-0038824016

Web Of Science Accession Number

Start Page

  • 101

End Page

  • 105

Volume

  • 17

Issue

  • 2