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3-Dimensional super-resolution by spectrally selective imaging

Journal Article


Abstract


  • The mutual spatial positions of individual pentacene molecules embedded in a p-terphenyl host crystal, residing within the same diffraction-limited volume, are determined with far-field optics with an accuracy far below the Rayleigh distance. This is achieved by spectrally selective imaging, an approach based on the combination of confocal microscopy, single-molecule detection and position-sensitive imaging. It is demonstrated that the molecules can be localized in three dimensions with a precision of 40 nm in the lateral and 100 nm in the axial dimension which represents an enhancement by a factor of 20 and 65, respectively, compared to the Rayleigh distance at the used numerical aperture of the microscope.

Publication Date


  • 1998

Citation


  • Van Oijen, A. M., Köhler, J., Schmidt, J., Müller, M., & Brakenhoff, G. J. (1998). 3-Dimensional super-resolution by spectrally selective imaging. Chemical Physics Letters, 292(1-2), 183-187. doi:10.1016/S0009-2614(98)00673-3

Scopus Eid


  • 2-s2.0-0032584419

Start Page


  • 183

End Page


  • 187

Volume


  • 292

Issue


  • 1-2

Abstract


  • The mutual spatial positions of individual pentacene molecules embedded in a p-terphenyl host crystal, residing within the same diffraction-limited volume, are determined with far-field optics with an accuracy far below the Rayleigh distance. This is achieved by spectrally selective imaging, an approach based on the combination of confocal microscopy, single-molecule detection and position-sensitive imaging. It is demonstrated that the molecules can be localized in three dimensions with a precision of 40 nm in the lateral and 100 nm in the axial dimension which represents an enhancement by a factor of 20 and 65, respectively, compared to the Rayleigh distance at the used numerical aperture of the microscope.

Publication Date


  • 1998

Citation


  • Van Oijen, A. M., Köhler, J., Schmidt, J., Müller, M., & Brakenhoff, G. J. (1998). 3-Dimensional super-resolution by spectrally selective imaging. Chemical Physics Letters, 292(1-2), 183-187. doi:10.1016/S0009-2614(98)00673-3

Scopus Eid


  • 2-s2.0-0032584419

Start Page


  • 183

End Page


  • 187

Volume


  • 292

Issue


  • 1-2