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Three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy

Conference Paper


Abstract


  • It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA=0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

Publication Date


  • 1999

Citation


  • Brakenhoff, G. J., van Oijen, A. M., Kohler, J., Muller, M., & Schmidt, J. (1999). Three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy. In Proceedings of SPIE - The International Society for Optical Engineering Vol. 3605 (pp. 82-89).

Scopus Eid


  • 2-s2.0-0032658493

Start Page


  • 82

End Page


  • 89

Volume


  • 3605

Abstract


  • It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA=0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

Publication Date


  • 1999

Citation


  • Brakenhoff, G. J., van Oijen, A. M., Kohler, J., Muller, M., & Schmidt, J. (1999). Three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy. In Proceedings of SPIE - The International Society for Optical Engineering Vol. 3605 (pp. 82-89).

Scopus Eid


  • 2-s2.0-0032658493

Start Page


  • 82

End Page


  • 89

Volume


  • 3605