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Spectroscopy of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila: Diagonal disorder, intercomplex heterogeneity, spectral diffusion, and energy transfer in the B800 band

Journal Article


Abstract


  • This paper reports a detailed spectroscopic study of the B800 absorption band of individual light-harvesting 2 (LH2) complexes of the photosynthetic purple bacterium Rhodopseudomonas acidophila at 1.2 K. By applying single- molecule detection techniques to this system, details and properties can be revealed that remain obscured in conventional ensemble experiments. For instance, from fluorescence-excitation spectra of the individual complexes a more direct measure of the diagonal disorder could be obtained. Further spectral diffusion phenomena and homogeneous linewidths of individual bacteriochlorophyll a (BChl a) molecules are observed, revealing valuable information on excited-state dynamics. This work demonstrates that it is possible to obtain detailed spectral information on individual pigment- protein complexes, providing direct insight into their electronic structure and into the mechanisms underlying the highly efficient energy transfer processes in these systems.

Publication Date


  • 2000

Citation


  • Van Oijen, A. M., Ketelaars, M., Köhler, J., Aartsma, T. J., & Schmidt, J. (2000). Spectroscopy of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila: Diagonal disorder, intercomplex heterogeneity, spectral diffusion, and energy transfer in the B800 band. Biophysical Journal, 78(3), 1570-1577. doi:10.1016/S0006-3495(00)76709-9

Scopus Eid


  • 2-s2.0-0034052967

Start Page


  • 1570

End Page


  • 1577

Volume


  • 78

Issue


  • 3

Abstract


  • This paper reports a detailed spectroscopic study of the B800 absorption band of individual light-harvesting 2 (LH2) complexes of the photosynthetic purple bacterium Rhodopseudomonas acidophila at 1.2 K. By applying single- molecule detection techniques to this system, details and properties can be revealed that remain obscured in conventional ensemble experiments. For instance, from fluorescence-excitation spectra of the individual complexes a more direct measure of the diagonal disorder could be obtained. Further spectral diffusion phenomena and homogeneous linewidths of individual bacteriochlorophyll a (BChl a) molecules are observed, revealing valuable information on excited-state dynamics. This work demonstrates that it is possible to obtain detailed spectral information on individual pigment- protein complexes, providing direct insight into their electronic structure and into the mechanisms underlying the highly efficient energy transfer processes in these systems.

Publication Date


  • 2000

Citation


  • Van Oijen, A. M., Ketelaars, M., Köhler, J., Aartsma, T. J., & Schmidt, J. (2000). Spectroscopy of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila: Diagonal disorder, intercomplex heterogeneity, spectral diffusion, and energy transfer in the B800 band. Biophysical Journal, 78(3), 1570-1577. doi:10.1016/S0006-3495(00)76709-9

Scopus Eid


  • 2-s2.0-0034052967

Start Page


  • 1570

End Page


  • 1577

Volume


  • 78

Issue


  • 3