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Crystallization of two glutathione S-transferases from an unusual gene family

Journal Article


Abstract


  • Two glutathione S-transferase isozymes from the mosquito Anopheles dirus (AdGST1-3 and AdGST1-4) from an alternately spliced gene family have been expressed, purified and crystallized. The isozymes share an N-terminal domain derived from a single exon and C-terminal domains from unique exons. Despite the high level of sequence identity (64% overall), the two isozymes crystallize in different space groups, the 1-3 isozyme in P3121 or P3221 (unit-cell parameters a = 49.9, c = 271.8 �� at 100 K) and the 1-4 isozyme in P41 or P43 (unit-cell parameters a = 87.8, c = 166.1 at 100 K). Determination of these structures will advance our understanding of how these enzymes inactivate pesticides and the structural consequences of alternate splicing.

Publication Date


  • 2001

Citation


  • Oakley, A. J., Jirajaroenrat, K., Harnnoi, T., Ketterman, A. J., & Wilce, M. C. J. (2001). Crystallization of two glutathione S-transferases from an unusual gene family. Acta Crystallographica Section D: Biological Crystallography, 57(6), 870-872. doi:10.1107/S0907444901004929

Scopus Eid


  • 2-s2.0-0034979951

Start Page


  • 870

End Page


  • 872

Volume


  • 57

Issue


  • 6

Place Of Publication


Abstract


  • Two glutathione S-transferase isozymes from the mosquito Anopheles dirus (AdGST1-3 and AdGST1-4) from an alternately spliced gene family have been expressed, purified and crystallized. The isozymes share an N-terminal domain derived from a single exon and C-terminal domains from unique exons. Despite the high level of sequence identity (64% overall), the two isozymes crystallize in different space groups, the 1-3 isozyme in P3121 or P3221 (unit-cell parameters a = 49.9, c = 271.8 �� at 100 K) and the 1-4 isozyme in P41 or P43 (unit-cell parameters a = 87.8, c = 166.1 at 100 K). Determination of these structures will advance our understanding of how these enzymes inactivate pesticides and the structural consequences of alternate splicing.

Publication Date


  • 2001

Citation


  • Oakley, A. J., Jirajaroenrat, K., Harnnoi, T., Ketterman, A. J., & Wilce, M. C. J. (2001). Crystallization of two glutathione S-transferases from an unusual gene family. Acta Crystallographica Section D: Biological Crystallography, 57(6), 870-872. doi:10.1107/S0907444901004929

Scopus Eid


  • 2-s2.0-0034979951

Start Page


  • 870

End Page


  • 872

Volume


  • 57

Issue


  • 6

Place Of Publication