Skip to main content
placeholder image

Evidence for an induced-fit mechanism operating in pi class glutathione transferases

Journal Article


Abstract


  • Three-dimensional structures of the apo form of human pi class glutathione transferase have been determined by X-ray crystallography. The structures suggest the enzyme recognizes its substrate, glutathione, by an induced-fit mechanism. Compared to complexed forms of the enzyme, the environment around the catalytic residue, Tyr 7, remains unchanged in the apoenzyme. This observation supports the view that Tyr 7 does not act as a general base in the reaction mechanism. The observed cooperativity of the dimeric enzyme may be due to the movements of a helix that forms one wall of the active site and, in particular, to movements of a tyrosine residue that is located in the subunit interface.

Publication Date


  • 1998

Citation


  • Oakley, A. J., Lo Bello, M., Ricci, G., Federici, G., & Parker, M. W. (1998). Evidence for an induced-fit mechanism operating in pi class glutathione transferases. Biochemistry, 37(28), 9912-9917. doi:10.1021/bi980323w

Scopus Eid


  • 2-s2.0-0032516446

Start Page


  • 9912

End Page


  • 9917

Volume


  • 37

Issue


  • 28

Abstract


  • Three-dimensional structures of the apo form of human pi class glutathione transferase have been determined by X-ray crystallography. The structures suggest the enzyme recognizes its substrate, glutathione, by an induced-fit mechanism. Compared to complexed forms of the enzyme, the environment around the catalytic residue, Tyr 7, remains unchanged in the apoenzyme. This observation supports the view that Tyr 7 does not act as a general base in the reaction mechanism. The observed cooperativity of the dimeric enzyme may be due to the movements of a helix that forms one wall of the active site and, in particular, to movements of a tyrosine residue that is located in the subunit interface.

Publication Date


  • 1998

Citation


  • Oakley, A. J., Lo Bello, M., Ricci, G., Federici, G., & Parker, M. W. (1998). Evidence for an induced-fit mechanism operating in pi class glutathione transferases. Biochemistry, 37(28), 9912-9917. doi:10.1021/bi980323w

Scopus Eid


  • 2-s2.0-0032516446

Start Page


  • 9912

End Page


  • 9917

Volume


  • 37

Issue


  • 28