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A crystallographic snapshot of tyrosine trans-phosphorylation in action

Journal Article


Abstract


  • Tyrosine trans-phosphorylation is a key event in receptor tyrosine kinase signaling, yet, the structural basis for this process has eluded definition. Here, we present the crystal structure of the FGF receptor 2 kinases caught in the act of trans-phosphorylation of Y769, the major C-terminal phosphorylation site. The structure reveals that enzyme- and substrate-acting kinases engage each other through elaborate and specific interactions not only in the immediate vicinity of Y769 and the enzyme active site, but also in regions that are as much of 18 Å away from D626, the catalytic base in the enzyme active site. These interactions lead to an unprecedented level of specificity and precision during the trans-phosphorylation on Y769. Time-resolved mass spectrometry analysis supports the observed mechanism of trans-phosphorylation. Our data provide a molecular framework for understanding the mechanism of action of Kallmann syndrome mutations and the order of trans-phosphorylation reactions in FGFRs. We propose that the salient mechanistic features of Y769 transphosphorylation are applicable to trans-phosphorylation of the equivalent major phosphorylation sites in many other RTKs. © 2008 by The National Academy of Sciences of the USA.

Publication Date


  • 2008

Citation


  • Chen, H., Xua, C. F., Ma, J., Eliseenkova, A. V., Li, W., Pollock, P. M., . . . Mohammadi, M. (2008). A crystallographic snapshot of tyrosine trans-phosphorylation in action. Proceedings of the National Academy of Sciences of the United States of America, 105(50), 19660-19665. doi:10.1073/pnas.0807752105

Scopus Eid


  • 2-s2.0-58149399336

Start Page


  • 19660

End Page


  • 19665

Volume


  • 105

Issue


  • 50

Abstract


  • Tyrosine trans-phosphorylation is a key event in receptor tyrosine kinase signaling, yet, the structural basis for this process has eluded definition. Here, we present the crystal structure of the FGF receptor 2 kinases caught in the act of trans-phosphorylation of Y769, the major C-terminal phosphorylation site. The structure reveals that enzyme- and substrate-acting kinases engage each other through elaborate and specific interactions not only in the immediate vicinity of Y769 and the enzyme active site, but also in regions that are as much of 18 Å away from D626, the catalytic base in the enzyme active site. These interactions lead to an unprecedented level of specificity and precision during the trans-phosphorylation on Y769. Time-resolved mass spectrometry analysis supports the observed mechanism of trans-phosphorylation. Our data provide a molecular framework for understanding the mechanism of action of Kallmann syndrome mutations and the order of trans-phosphorylation reactions in FGFRs. We propose that the salient mechanistic features of Y769 transphosphorylation are applicable to trans-phosphorylation of the equivalent major phosphorylation sites in many other RTKs. © 2008 by The National Academy of Sciences of the USA.

Publication Date


  • 2008

Citation


  • Chen, H., Xua, C. F., Ma, J., Eliseenkova, A. V., Li, W., Pollock, P. M., . . . Mohammadi, M. (2008). A crystallographic snapshot of tyrosine trans-phosphorylation in action. Proceedings of the National Academy of Sciences of the United States of America, 105(50), 19660-19665. doi:10.1073/pnas.0807752105

Scopus Eid


  • 2-s2.0-58149399336

Start Page


  • 19660

End Page


  • 19665

Volume


  • 105

Issue


  • 50