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pH-Controlled quaternary states of hexameric DnaB helicase

Journal Article


Abstract


  • DnaB is the major helicase in the Escherichia coli replisome. It is a homohexameric enzyme that interacts with many other replisomal proteins and cofactors. It is usually loaded onto a single strand of DNA at origins of replication from its complex with its loading partner DnaC, then translocates in the 5' to 3' direction, unwinding duplex DNA in an NTP-driven process. Quaternary polymorphism has been described for the DnaB oligomer, a feature it has in common with some other hexameric helicases. In the present work, electron microscopy and indepth rotational analysis studies of negatively stained specimens has allowed the establishment of conditions that govern the transition between the two different rotational symmetry states (C3 and C6) of DnaB. It is shown: (a) that the pH value of the sample buffer, within the physiological range, dictates the quaternary organisation of the DnaB oligomer; (b) that the pH-induced transition is fully reversible; (c) that the type of adenine nucleotide complexed to DnaB, whether hydrolysable or not, does not affect its quaternary architecture; (d) that the DnaB · DnaC complex exists only as particles with C3 symmetry; and (e) that DnaC interacts only with DnaB particles that have C3 symmetry. Structural consequences of this quaternary polymorphism, as well as its functional implications for helicase activity, are discussed. (C) 2000 Academic Press.

Publication Date


  • 2000

Citation


  • Donate, L. E., Llorca, O., Bárcena, M., Brown, S. E., Dixon, N. E., & Carazo, J. M. (2000). pH-Controlled quaternary states of hexameric DnaB helicase. Journal of Molecular Biology, 303(3), 383-393. doi:10.1006/jmbi.2000.4132

Scopus Eid


  • 2-s2.0-0034721948

Start Page


  • 383

End Page


  • 393

Volume


  • 303

Issue


  • 3

Abstract


  • DnaB is the major helicase in the Escherichia coli replisome. It is a homohexameric enzyme that interacts with many other replisomal proteins and cofactors. It is usually loaded onto a single strand of DNA at origins of replication from its complex with its loading partner DnaC, then translocates in the 5' to 3' direction, unwinding duplex DNA in an NTP-driven process. Quaternary polymorphism has been described for the DnaB oligomer, a feature it has in common with some other hexameric helicases. In the present work, electron microscopy and indepth rotational analysis studies of negatively stained specimens has allowed the establishment of conditions that govern the transition between the two different rotational symmetry states (C3 and C6) of DnaB. It is shown: (a) that the pH value of the sample buffer, within the physiological range, dictates the quaternary organisation of the DnaB oligomer; (b) that the pH-induced transition is fully reversible; (c) that the type of adenine nucleotide complexed to DnaB, whether hydrolysable or not, does not affect its quaternary architecture; (d) that the DnaB · DnaC complex exists only as particles with C3 symmetry; and (e) that DnaC interacts only with DnaB particles that have C3 symmetry. Structural consequences of this quaternary polymorphism, as well as its functional implications for helicase activity, are discussed. (C) 2000 Academic Press.

Publication Date


  • 2000

Citation


  • Donate, L. E., Llorca, O., Bárcena, M., Brown, S. E., Dixon, N. E., & Carazo, J. M. (2000). pH-Controlled quaternary states of hexameric DnaB helicase. Journal of Molecular Biology, 303(3), 383-393. doi:10.1006/jmbi.2000.4132

Scopus Eid


  • 2-s2.0-0034721948

Start Page


  • 383

End Page


  • 393

Volume


  • 303

Issue


  • 3