A novel concept of dielectrophoresis (DEP)-assisted hydrophoretic focusing of micro-particles is presented in this investigation. The DEP-assisted hydrophoretic micro-channel consists of crescent-shaped grooves and interdigitated electrodes. The crescent-shaped grooves was introduced to generate lateral pressure gradients that induce helical recirculations. A negative DEP force induced by interdigitated electrodes pushes the micro-particles into a higher level in the micro-channel. Particles passing through the microfluidic device are focused onto the sidewalls of the channel. In this device, both numerical and experimental particle trajectories were investigated and the focusing pattern of particles can be tuned by changing the external voltages.