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Effective detection of corrected dystrophin loci in mdx mouse myogenic precursors

Journal Article


Abstract


  • Targeted corrective gene conversion (TCGC) holds much promise as a future therapy for many hereditary diseases in humans. Mutation correction frequencies varying between 0.0001% and 40% have been reported using chimeraplasty, oligoplasty, triplex-forming oligonucleotides, and small corrective PCR amplicons (CPA). However, PCR technologies used to detect correction events risk either falsely indicating or greatly exaggerating the presence of corrected loci. This is a problem that is considerably exacerbated by attempted improvement of the TCGC system using high corrective nucleic acid (CNA) to nuclear ratios. Small fragment homologous replacement (SFHR)-mediated correction of the exon 23 dystrophin (DMD) gene mutation in the mdx mouse model of DMD has been used in this study to evaluate the effect of increasing CPA amounts. In these experiments, we detected extremely high levels of apparently corrected loci and determined that at higher CNA to nuclear ratios the extent of locus correction was highly exaggerated by residual CNA species in the nucleic acids extracted from the treated cells. This study describes a generic locus-specific detection protocol designed to eradicate residual CNA species and avoid the artifactual or exaggerated detection of gene correction. © 2007 Wiley-Liss, Inc.

UOW Authors


  •   Kapsa, Robert
  •   Kita, Magdalena (external author)
  •   Quigley, Anita (external author)

Publication Date


  • 2007

Citation


  • Todaro, M., Quigley, A., Kita, M., Chin, J., Lowes, K., Kornberg, A. J., . . . Kapsa, R. (2007). Effective detection of corrected dystrophin loci in mdx mouse myogenic precursors. Human Mutation, 28(8), 816-823. doi:10.1002/humu.20494

Scopus Eid


  • 2-s2.0-34548012643

Start Page


  • 816

End Page


  • 823

Volume


  • 28

Issue


  • 8

Abstract


  • Targeted corrective gene conversion (TCGC) holds much promise as a future therapy for many hereditary diseases in humans. Mutation correction frequencies varying between 0.0001% and 40% have been reported using chimeraplasty, oligoplasty, triplex-forming oligonucleotides, and small corrective PCR amplicons (CPA). However, PCR technologies used to detect correction events risk either falsely indicating or greatly exaggerating the presence of corrected loci. This is a problem that is considerably exacerbated by attempted improvement of the TCGC system using high corrective nucleic acid (CNA) to nuclear ratios. Small fragment homologous replacement (SFHR)-mediated correction of the exon 23 dystrophin (DMD) gene mutation in the mdx mouse model of DMD has been used in this study to evaluate the effect of increasing CPA amounts. In these experiments, we detected extremely high levels of apparently corrected loci and determined that at higher CNA to nuclear ratios the extent of locus correction was highly exaggerated by residual CNA species in the nucleic acids extracted from the treated cells. This study describes a generic locus-specific detection protocol designed to eradicate residual CNA species and avoid the artifactual or exaggerated detection of gene correction. © 2007 Wiley-Liss, Inc.

UOW Authors


  •   Kapsa, Robert
  •   Kita, Magdalena (external author)
  •   Quigley, Anita (external author)

Publication Date


  • 2007

Citation


  • Todaro, M., Quigley, A., Kita, M., Chin, J., Lowes, K., Kornberg, A. J., . . . Kapsa, R. (2007). Effective detection of corrected dystrophin loci in mdx mouse myogenic precursors. Human Mutation, 28(8), 816-823. doi:10.1002/humu.20494

Scopus Eid


  • 2-s2.0-34548012643

Start Page


  • 816

End Page


  • 823

Volume


  • 28

Issue


  • 8