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A new microsphere-based immunofluorescence assay using flow cytometry

Journal Article


Abstract


  • The quantitative and qualitative capacities of flow cytometric analysis that have made it such a powerful tool in studies of cellular antigens have not previously been exploited when dealing with non-cellular antigens. A new immunofluorescence assay technique was developed, using an indirect staining procedure with monoclonal anti-�� antibodies, to detect human free �� light chains covalently bound to microspheres of a size suitable for flow cytometry. The strength of the fluorescent signal produced on the microspheres was related to the amount of antigen bound and the size of the beads. At the time of this work large microspheres (i.e., greater than 3 ��m in diameter) suitable for this application were only available as suspensions of polysized beads. The fluorescent signal detected on labelled beads was optimized by selecting for analysis, on the basis of the forwrad angle laser scatter, only those beads of largest diameter. There are many potential applications for this technique - microspheres can be used for the presentation of virtually any antigen or antibody. The analytical benefits inherent in flow cytometry would be a significant advantage in the development of quantitative assays using this method. �� 1988.

Publication Date


  • 1988

Citation


  • Wilson, M. R., & Wotherspoon, J. S. (1988). A new microsphere-based immunofluorescence assay using flow cytometry. Journal of Immunological Methods, 107(2), 225-230. doi:10.1016/0022-1759(88)90222-0

Scopus Eid


  • 2-s2.0-0023872196

Start Page


  • 225

End Page


  • 230

Volume


  • 107

Issue


  • 2

Place Of Publication


Abstract


  • The quantitative and qualitative capacities of flow cytometric analysis that have made it such a powerful tool in studies of cellular antigens have not previously been exploited when dealing with non-cellular antigens. A new immunofluorescence assay technique was developed, using an indirect staining procedure with monoclonal anti-�� antibodies, to detect human free �� light chains covalently bound to microspheres of a size suitable for flow cytometry. The strength of the fluorescent signal produced on the microspheres was related to the amount of antigen bound and the size of the beads. At the time of this work large microspheres (i.e., greater than 3 ��m in diameter) suitable for this application were only available as suspensions of polysized beads. The fluorescent signal detected on labelled beads was optimized by selecting for analysis, on the basis of the forwrad angle laser scatter, only those beads of largest diameter. There are many potential applications for this technique - microspheres can be used for the presentation of virtually any antigen or antibody. The analytical benefits inherent in flow cytometry would be a significant advantage in the development of quantitative assays using this method. �� 1988.

Publication Date


  • 1988

Citation


  • Wilson, M. R., & Wotherspoon, J. S. (1988). A new microsphere-based immunofluorescence assay using flow cytometry. Journal of Immunological Methods, 107(2), 225-230. doi:10.1016/0022-1759(88)90222-0

Scopus Eid


  • 2-s2.0-0023872196

Start Page


  • 225

End Page


  • 230

Volume


  • 107

Issue


  • 2

Place Of Publication