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Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells

Journal Article


Abstract


  • Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.

UOW Authors


  •   Tang, Shiyang (external author)

Publication Date


  • 2017

Citation


  • Cole, R. H., Tang, S. Y., Siltanen, C. A., Shahi, P., Zhang, J. Q., Poust, S., . . . Abate, A. R. (2017). Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells. Proceedings of the National Academy of Sciences of the United States of America, 114(33), 8728-8733. doi:10.1073/pnas.1704020114

Scopus Eid


  • 2-s2.0-85027421088

Start Page


  • 8728

End Page


  • 8733

Volume


  • 114

Issue


  • 33

Abstract


  • Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.

UOW Authors


  •   Tang, Shiyang (external author)

Publication Date


  • 2017

Citation


  • Cole, R. H., Tang, S. Y., Siltanen, C. A., Shahi, P., Zhang, J. Q., Poust, S., . . . Abate, A. R. (2017). Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells. Proceedings of the National Academy of Sciences of the United States of America, 114(33), 8728-8733. doi:10.1073/pnas.1704020114

Scopus Eid


  • 2-s2.0-85027421088

Start Page


  • 8728

End Page


  • 8733

Volume


  • 114

Issue


  • 33