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Phytochemical and biological activity studies of the Bhutanese medicinal plant corydalis crispa

Journal Article


Abstract


  • The chemical constituents and biological activities of Corydalis crispa (Fumariaceae) were investigated for the first time. The phytochemical study resulted in the isolation of nine known isoquinoline alkaloids: protopine (1), 13-oxoprotopine (2), 13-oxocryptopine (3), stylopine (4), coreximine (5), rheagenine (6), ochrobirine (7), sibiricine (8) and bicuculline (9), with complete NMR data for 2 and 3 provided here for the first time. Crude extracts exhibited significant anti-inflammatory (p<0.01) activity against TNF-α production in LPS activated THP-1 cells. The acetylcholinesterase inhibitory activity of compounds 2, 4 and 7 and the antiplasmodial activity of compound 5 against P. falciparum strains TM4/8.2 and K1CB1 (multidrug resistant strain) are reported here for the first time. Stylopine (4) did not show antimalarial activity against the K1CB1 strain in contrast to a previous report. This study generated a scientific basis for the use of this plant in Bhutanese traditional medicine, either individually or in combination with other medicinal ingredients to treat a broad range of disorders. This study also identified compound 5 as potential new antimalarial lead compound.

Publication Date


  • 2012

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Sastraruji, T., Taweechotipatr, M., Rattanajak, R., . . . Kamchonwongpaisan, S. (2012). Phytochemical and biological activity studies of the Bhutanese medicinal plant corydalis crispa. Natural Product Communications, 7(5), 575-580. doi:10.1177/1934578x1200700507

Scopus Eid


  • 2-s2.0-84861505544

Start Page


  • 575

End Page


  • 580

Volume


  • 7

Issue


  • 5

Abstract


  • The chemical constituents and biological activities of Corydalis crispa (Fumariaceae) were investigated for the first time. The phytochemical study resulted in the isolation of nine known isoquinoline alkaloids: protopine (1), 13-oxoprotopine (2), 13-oxocryptopine (3), stylopine (4), coreximine (5), rheagenine (6), ochrobirine (7), sibiricine (8) and bicuculline (9), with complete NMR data for 2 and 3 provided here for the first time. Crude extracts exhibited significant anti-inflammatory (p<0.01) activity against TNF-α production in LPS activated THP-1 cells. The acetylcholinesterase inhibitory activity of compounds 2, 4 and 7 and the antiplasmodial activity of compound 5 against P. falciparum strains TM4/8.2 and K1CB1 (multidrug resistant strain) are reported here for the first time. Stylopine (4) did not show antimalarial activity against the K1CB1 strain in contrast to a previous report. This study generated a scientific basis for the use of this plant in Bhutanese traditional medicine, either individually or in combination with other medicinal ingredients to treat a broad range of disorders. This study also identified compound 5 as potential new antimalarial lead compound.

Publication Date


  • 2012

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Sastraruji, T., Taweechotipatr, M., Rattanajak, R., . . . Kamchonwongpaisan, S. (2012). Phytochemical and biological activity studies of the Bhutanese medicinal plant corydalis crispa. Natural Product Communications, 7(5), 575-580. doi:10.1177/1934578x1200700507

Scopus Eid


  • 2-s2.0-84861505544

Start Page


  • 575

End Page


  • 580

Volume


  • 7

Issue


  • 5