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GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile

Journal Article


Abstract


  • We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Taweechotipatr, M., & Kamchonwongpaisan, S. (2013). GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile. Natural Product Communications, 8(9), 1305-1308. doi:10.1177/1934578x1300800930

Scopus Eid


  • 2-s2.0-84886805648

Start Page


  • 1305

End Page


  • 1308

Volume


  • 8

Issue


  • 9

Abstract


  • We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Taweechotipatr, M., & Kamchonwongpaisan, S. (2013). GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile. Natural Product Communications, 8(9), 1305-1308. doi:10.1177/1934578x1300800930

Scopus Eid


  • 2-s2.0-84886805648

Start Page


  • 1305

End Page


  • 1308

Volume


  • 8

Issue


  • 9