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Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system

Journal Article


Abstract


  • We investigated the gap junctional properties of human embryonic stem cells (hESC) cultivated in a serum-free system using sphingosine-1-phosphate and platelet-derived growth factor (S1P/PDGF). We compared this condition to hESC grown on Matrigel in mouse embryonic fibroblast conditioned medium (MEF-CM) or unconditioned medium (UM). We show that in all culture systems, hESC express connexins 43 and 45. hESC maintained in S1P/PDGF conditions and hESC grown in presence of MEF-CM are coupled through gap junctions while hESC maintained on Matrigel in UM do not exhibit gap junctional intercellular communication. In this latter condition, coupling was retrieved by addition of noggin, suggesting that BMP-like activity in UM inhibits gap junctional communication. Last, our data indicate that the closure of gap junctions by the decoupling agent α-glycyrrhetinic acid increases cell apoptosis and inhibits hESC colony growth. Altogether, these results suggest that gap junctions play an important role in hESC maintenance. © 2006 Elsevier Inc. All rights reserved.

Publication Date


  • 2006

Citation


  • Wong, R. C. B., Dottori, M., Koh, K. L. L., Nguyen, L. T. V., Pera, M. F., & Pébay, A. (2006). Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system. Biochemical and Biophysical Research Communications, 344(1), 181-188. doi:10.1016/j.bbrc.2006.03.127

Scopus Eid


  • 2-s2.0-33646065973

Start Page


  • 181

End Page


  • 188

Volume


  • 344

Issue


  • 1

Abstract


  • We investigated the gap junctional properties of human embryonic stem cells (hESC) cultivated in a serum-free system using sphingosine-1-phosphate and platelet-derived growth factor (S1P/PDGF). We compared this condition to hESC grown on Matrigel in mouse embryonic fibroblast conditioned medium (MEF-CM) or unconditioned medium (UM). We show that in all culture systems, hESC express connexins 43 and 45. hESC maintained in S1P/PDGF conditions and hESC grown in presence of MEF-CM are coupled through gap junctions while hESC maintained on Matrigel in UM do not exhibit gap junctional intercellular communication. In this latter condition, coupling was retrieved by addition of noggin, suggesting that BMP-like activity in UM inhibits gap junctional communication. Last, our data indicate that the closure of gap junctions by the decoupling agent α-glycyrrhetinic acid increases cell apoptosis and inhibits hESC colony growth. Altogether, these results suggest that gap junctions play an important role in hESC maintenance. © 2006 Elsevier Inc. All rights reserved.

Publication Date


  • 2006

Citation


  • Wong, R. C. B., Dottori, M., Koh, K. L. L., Nguyen, L. T. V., Pera, M. F., & Pébay, A. (2006). Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system. Biochemical and Biophysical Research Communications, 344(1), 181-188. doi:10.1016/j.bbrc.2006.03.127

Scopus Eid


  • 2-s2.0-33646065973

Start Page


  • 181

End Page


  • 188

Volume


  • 344

Issue


  • 1