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EphA4 is not required for Purkinje cell compartmentation

Journal Article


Abstract


  • The Purkinje cells of both the adult and the developing cerebellar cortex are organized into parasagittal stripes or 'segments' expressing a variety of biochemical markers. We show that in the developing mouse cerebellar cortex, members of the Eph receptor gene family are expressed in mediolaterally alternating Purkinje cell segments. Since members of the Eph receptors family have been shown to play a role in hindbrain segmentation and boundary formation (Philos. Trans. R. Soc. Lond. B: Biol. Sci. 355 (2000) 993), we analyzed the effect of a null mutation of the EphA4 gene on Purkinje cell compartmentation. Using well characterized markers of Purkinje cell compartmentation in both the developing and the adult cerebellum, we observed no significant alteration in the banding pattern of these markers between the EphA4 knockout mice and their wild type controls. The ribboned pattern of migrating granule cells in the developing cerebellum also appears unaltered. The expression of other members of this gene family, including ephrin-B2, EphA2, and ephrin-A1, in a compartmentalized pattern within the Purkinje cell layer suggests a possible redundancy and/or a compensation of EphA4 function in the segmental patterning of cerebellar Purkinje cells. © 2002 Elsevier Science B.V. All rights reserved.

Publication Date


  • 2002

Citation


  • Karam, S. D., Dottori, M., Ogawa, K., Henderson, J. T., Boyd, A. W., Pasquale, E. B., & Bothwell, M. (2002). EphA4 is not required for Purkinje cell compartmentation. Developmental Brain Research, 135(1-2), 29-38. doi:10.1016/S0165-3806(02)00278-X

Scopus Eid


  • 2-s2.0-0037197662

Start Page


  • 29

End Page


  • 38

Volume


  • 135

Issue


  • 1-2

Abstract


  • The Purkinje cells of both the adult and the developing cerebellar cortex are organized into parasagittal stripes or 'segments' expressing a variety of biochemical markers. We show that in the developing mouse cerebellar cortex, members of the Eph receptor gene family are expressed in mediolaterally alternating Purkinje cell segments. Since members of the Eph receptors family have been shown to play a role in hindbrain segmentation and boundary formation (Philos. Trans. R. Soc. Lond. B: Biol. Sci. 355 (2000) 993), we analyzed the effect of a null mutation of the EphA4 gene on Purkinje cell compartmentation. Using well characterized markers of Purkinje cell compartmentation in both the developing and the adult cerebellum, we observed no significant alteration in the banding pattern of these markers between the EphA4 knockout mice and their wild type controls. The ribboned pattern of migrating granule cells in the developing cerebellum also appears unaltered. The expression of other members of this gene family, including ephrin-B2, EphA2, and ephrin-A1, in a compartmentalized pattern within the Purkinje cell layer suggests a possible redundancy and/or a compensation of EphA4 function in the segmental patterning of cerebellar Purkinje cells. © 2002 Elsevier Science B.V. All rights reserved.

Publication Date


  • 2002

Citation


  • Karam, S. D., Dottori, M., Ogawa, K., Henderson, J. T., Boyd, A. W., Pasquale, E. B., & Bothwell, M. (2002). EphA4 is not required for Purkinje cell compartmentation. Developmental Brain Research, 135(1-2), 29-38. doi:10.1016/S0165-3806(02)00278-X

Scopus Eid


  • 2-s2.0-0037197662

Start Page


  • 29

End Page


  • 38

Volume


  • 135

Issue


  • 1-2