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Biological functions of GCS3, a novel plasminogen-binding protein of Streptococcus dysgalactiae ssp. equisimilis

Journal Article


Abstract


  • Increasing awareness of the relevance of Streptococcus dysgalactiae ssp. equisimilis as a human pathogen motivates the analysis of its pathomechanisms. One of the mechanisms that increases infectivity and dissemination of several streptococcal species is the recruitment and subsequent activation of host plasminogen on the streptococcal surface. This study identified GCS3 as a novel plasminogen-binding M protein of S. dysgalactiae ssp. equisimilis and revealed a difference in the mode of binding as compared to the plasminogen-binding protein PAM of S. pyogenes. In contrast to PAM, GCS3 did not bind to the kringle 1-3 region of plasminogen. Despite this difference, GCS3 exerts the same function of recruiting plasminogen to the streptococcal surface, which can be activated by streptokinase and host plasminogen activators to serve as a spreading factor. Moreover, we demonstrate a role of GCS3 in plasminogen-dependent streptococcal adherence to human pharyngeal cells (cell line Detroit 562) that indicates an additional function of the protein as an adhesin in the oral cavity.

Authors


  •   Bergmann, Rene (external author)
  •   Dinkla, Katrin (external author)
  •   Nitsche-Schmitz, D. Patric (external author)
  •   Graham, Rikki M. A. (external author)
  •   Luttge, Melanie (external author)
  •   Sanderson-Smith, Martina L.
  •   Nerlich, Andreas (external author)
  •   Rohde, Manfred (external author)
  •   Chhatwal, G S. (external author)

Publication Date


  • 2011

Geographic Focus


Citation


  • Bergmann, R., Dinkla, K., Nitsche-Schmitz, D., Graham, R. M. A., Luttge, M., Sanderson-Smith, M. L., Nerlich, A., Rohde, M. & Chhatwal, G. S. (2011). Biological functions of GCS3, a novel plasminogen-binding protein of Streptococcus dysgalactiae ssp. equisimilis. International Journal of Medical Microbiology, 301 (2), 157-164.

Scopus Eid


  • 2-s2.0-78751581649

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2646

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 157

End Page


  • 164

Volume


  • 301

Issue


  • 2

Place Of Publication


  • Germany

Abstract


  • Increasing awareness of the relevance of Streptococcus dysgalactiae ssp. equisimilis as a human pathogen motivates the analysis of its pathomechanisms. One of the mechanisms that increases infectivity and dissemination of several streptococcal species is the recruitment and subsequent activation of host plasminogen on the streptococcal surface. This study identified GCS3 as a novel plasminogen-binding M protein of S. dysgalactiae ssp. equisimilis and revealed a difference in the mode of binding as compared to the plasminogen-binding protein PAM of S. pyogenes. In contrast to PAM, GCS3 did not bind to the kringle 1-3 region of plasminogen. Despite this difference, GCS3 exerts the same function of recruiting plasminogen to the streptococcal surface, which can be activated by streptokinase and host plasminogen activators to serve as a spreading factor. Moreover, we demonstrate a role of GCS3 in plasminogen-dependent streptococcal adherence to human pharyngeal cells (cell line Detroit 562) that indicates an additional function of the protein as an adhesin in the oral cavity.

Authors


  •   Bergmann, Rene (external author)
  •   Dinkla, Katrin (external author)
  •   Nitsche-Schmitz, D. Patric (external author)
  •   Graham, Rikki M. A. (external author)
  •   Luttge, Melanie (external author)
  •   Sanderson-Smith, Martina L.
  •   Nerlich, Andreas (external author)
  •   Rohde, Manfred (external author)
  •   Chhatwal, G S. (external author)

Publication Date


  • 2011

Geographic Focus


Citation


  • Bergmann, R., Dinkla, K., Nitsche-Schmitz, D., Graham, R. M. A., Luttge, M., Sanderson-Smith, M. L., Nerlich, A., Rohde, M. & Chhatwal, G. S. (2011). Biological functions of GCS3, a novel plasminogen-binding protein of Streptococcus dysgalactiae ssp. equisimilis. International Journal of Medical Microbiology, 301 (2), 157-164.

Scopus Eid


  • 2-s2.0-78751581649

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2646

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 157

End Page


  • 164

Volume


  • 301

Issue


  • 2

Place Of Publication


  • Germany