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Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility

Journal Article


Abstract


  • Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling ACOOH groups on PLA membranes and ANH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600-3000 cm 1 and 1700-1500 cm 1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility. © 2013 Elsevier Ltd. All rights reserved.

UOW Authors


  •   Nyanhongo, Gibson S. (external author)
  •   Rodríguez, Rosario D. (external author)
  •   Prasetyo, Endry N. (external author)
  •   Caparrós, Cristina (external author)
  •   Ribeiro, Clarisse (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Lanceros-Méndez, Senentxu (external author)
  •   Herrero Acero, Enrique (external author)
  •   Güebitz, Georg M. (external author)

Publication Date


  • 2013

Citation


  • Nyanhongo, G. S., Rodríguez, R. Diaz., Prasetyo, E. Nugroho., Caparrós, C., Ribeiro, C., Sencadas, V., Lanceros-Méndez, S., Herrero Acero, E. & Güebitz, G. M. (2013). Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility. Reactive and Functional Polymers, 73 (10), 1399-1404.

Scopus Eid


  • 2-s2.0-84885382734

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/3697

Number Of Pages


  • 5

Start Page


  • 1399

End Page


  • 1404

Volume


  • 73

Issue


  • 10

Abstract


  • Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling ACOOH groups on PLA membranes and ANH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600-3000 cm 1 and 1700-1500 cm 1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility. © 2013 Elsevier Ltd. All rights reserved.

UOW Authors


  •   Nyanhongo, Gibson S. (external author)
  •   Rodríguez, Rosario D. (external author)
  •   Prasetyo, Endry N. (external author)
  •   Caparrós, Cristina (external author)
  •   Ribeiro, Clarisse (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Lanceros-Méndez, Senentxu (external author)
  •   Herrero Acero, Enrique (external author)
  •   Güebitz, Georg M. (external author)

Publication Date


  • 2013

Citation


  • Nyanhongo, G. S., Rodríguez, R. Diaz., Prasetyo, E. Nugroho., Caparrós, C., Ribeiro, C., Sencadas, V., Lanceros-Méndez, S., Herrero Acero, E. & Güebitz, G. M. (2013). Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility. Reactive and Functional Polymers, 73 (10), 1399-1404.

Scopus Eid


  • 2-s2.0-84885382734

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/3697

Number Of Pages


  • 5

Start Page


  • 1399

End Page


  • 1404

Volume


  • 73

Issue


  • 10