A hybrid dielectrophoretic and hydrophoretic microchip for particle sorting using integrated pre-focusing and sorting steps

This work explores dielectrophoresis (DEP)-active hydrophoresis in sorting particles and

cells. The device consists of prefocusing region and sorting region with great potential

to be integrated into advanced lab-on-a-chip bioanalysis devices. Particles or cells can be

focused in the prefocusing region and then sorted in the sorting region. The DEP-active

hydrophoretic sorting is not only based on size but also on dielectric properties of the

particles or cells of interest without any labelling. A mixture of 3 and 10 um particles

were sorted and collected from corresponding outlets with high separation efficiency.

According to the different dielectric properties of viable and nonviable Chinese Hamster

Ovary (CHO) cells at the medium conductivity of 0.03 S/m, the viable CHO cells were

focused well and sorted from cell sample with a high purity.