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A hybrid dielectrophoretic and hydrophoretic microchip for particle sorting using integrated pre-focusing and sorting steps

Journal Article


Abstract


  • This work explores dielectrophoresis (DEP)-active hydrophoresis in sorting particles and

    cells. The device consists of prefocusing region and sorting region with great potential

    to be integrated into advanced lab-on-a-chip bioanalysis devices. Particles or cells can be

    focused in the prefocusing region and then sorted in the sorting region. The DEP-active

    hydrophoretic sorting is not only based on size but also on dielectric properties of the

    particles or cells of interest without any labelling. A mixture of 3 and 10 um particles

    were sorted and collected from corresponding outlets with high separation efficiency.

    According to the different dielectric properties of viable and nonviable Chinese Hamster

    Ovary (CHO) cells at the medium conductivity of 0.03 S/m, the viable CHO cells were

    focused well and sorted from cell sample with a high purity.

Publication Date


  • 2015

Citation


  • Yan, S., Zhang, J., Yuan, Y., Lovrecz, G., Alici, G., Du, H., Zhu, Y. & Li, W. (2015). A hybrid dielectrophoretic and hydrophoretic microchip for particle sorting using integrated pre-focusing and sorting steps. Electrophoresis, 36 (2), 284-291.

Scopus Eid


  • 2-s2.0-84921511015

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/3432

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 284

End Page


  • 291

Volume


  • 36

Issue


  • 2

Place Of Publication


  • Germany

Abstract


  • This work explores dielectrophoresis (DEP)-active hydrophoresis in sorting particles and

    cells. The device consists of prefocusing region and sorting region with great potential

    to be integrated into advanced lab-on-a-chip bioanalysis devices. Particles or cells can be

    focused in the prefocusing region and then sorted in the sorting region. The DEP-active

    hydrophoretic sorting is not only based on size but also on dielectric properties of the

    particles or cells of interest without any labelling. A mixture of 3 and 10 um particles

    were sorted and collected from corresponding outlets with high separation efficiency.

    According to the different dielectric properties of viable and nonviable Chinese Hamster

    Ovary (CHO) cells at the medium conductivity of 0.03 S/m, the viable CHO cells were

    focused well and sorted from cell sample with a high purity.

Publication Date


  • 2015

Citation


  • Yan, S., Zhang, J., Yuan, Y., Lovrecz, G., Alici, G., Du, H., Zhu, Y. & Li, W. (2015). A hybrid dielectrophoretic and hydrophoretic microchip for particle sorting using integrated pre-focusing and sorting steps. Electrophoresis, 36 (2), 284-291.

Scopus Eid


  • 2-s2.0-84921511015

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/3432

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 284

End Page


  • 291

Volume


  • 36

Issue


  • 2

Place Of Publication


  • Germany