This work explores dielectrophoresis (DEP)-active hydrophoresis in sorting particles and
cells. The device consists of prefocusing region and sorting region with great potential
to be integrated into advanced lab-on-a-chip bioanalysis devices. Particles or cells can be
focused in the prefocusing region and then sorted in the sorting region. The DEP-active
hydrophoretic sorting is not only based on size but also on dielectric properties of the
particles or cells of interest without any labelling. A mixture of 3 and 10 um particles
were sorted and collected from corresponding outlets with high separation efficiency.
According to the different dielectric properties of viable and nonviable Chinese Hamster
Ovary (CHO) cells at the medium conductivity of 0.03 S/m, the viable CHO cells were
focused well and sorted from cell sample with a high purity.