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An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

Journal Article


Abstract


  • In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by “cherry-picking” of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.

UOW Authors


  •   Burger, Robert (external author)
  •   Kurzbuch, D (external author)
  •   Gorkin III, Robert
  •   Kijanka, Gregor (external author)
  •   Glynn, Macdara (external author)
  •   Mcdonagh, C (external author)
  •   Ducree, Jens (external author)

Publication Date


  • 2015

Citation


  • Burger, R., Kurzbuch, D., Gorkin, R., Kijanka, G., Glynn, M., McDonagh, C. & Ducree, J. (2015). An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells. Lab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering, 15 (2), 378-381.

Scopus Eid


  • 2-s2.0-84918832285

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/1283

Has Global Citation Frequency


Number Of Pages


  • 3

Start Page


  • 378

End Page


  • 381

Volume


  • 15

Issue


  • 2

Place Of Publication


  • United Kingdom

Abstract


  • In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by “cherry-picking” of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.

UOW Authors


  •   Burger, Robert (external author)
  •   Kurzbuch, D (external author)
  •   Gorkin III, Robert
  •   Kijanka, Gregor (external author)
  •   Glynn, Macdara (external author)
  •   Mcdonagh, C (external author)
  •   Ducree, Jens (external author)

Publication Date


  • 2015

Citation


  • Burger, R., Kurzbuch, D., Gorkin, R., Kijanka, G., Glynn, M., McDonagh, C. & Ducree, J. (2015). An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells. Lab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering, 15 (2), 378-381.

Scopus Eid


  • 2-s2.0-84918832285

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/1283

Has Global Citation Frequency


Number Of Pages


  • 3

Start Page


  • 378

End Page


  • 381

Volume


  • 15

Issue


  • 2

Place Of Publication


  • United Kingdom