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Replication-fork dynamics

Chapter


Abstract


  • The proliferation of all organisms depends on the coordination of enzymatic events within

    large multiprotein replisomes that duplicate chromosomes. Whereas the structure and function

    of many core replisome components have been clarified, the timing and order of molecular

    events during replication remains obscure. To better understand the replication

    mechanism, new methods must be developed that allow for the observation and characterization

    of short-lived states and dynamic events at single replication forks. Over the last

    decade, great progress has been made toward this goal with the development of novel

    DNA nanomanipulation and fluorescence imaging techniques allowing for the direct observation

    of replication-fork dynamics both reconstituted in vitro and in live cells. This article

    reviews these new single-molecule approaches and the revised understanding of replisome

    operation that has emerged.

Authors


  •   Duderstadt, Karl E. (external author)
  •   Reyes-Lamothe, Rodrigo (external author)
  •   van Oijen, Antoine M.
  •   Sherratt, David J. (external author)

Publication Date


  • 2013

Citation


  • Duderstadt, K. E., Reyes-Lamothe, R., van Oijen, A. M. & Sherratt, D. J. (2013). Replication-fork dynamics. In S. Bell, M. Mechali & M. L. Depamphilis (Eds.), DNA Replication: a Subject Collection from Cold Spring Harbor Perspectives in Biology (pp. 147-163). New York: Cold Spring Harbor Laboratory Press.

Scopus Eid


  • 2-s2.0-84891649417

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2279

Book Title


  • DNA Replication: a Subject Collection from Cold Spring Harbor Perspectives in Biology

Has Global Citation Frequency


Start Page


  • 147

End Page


  • 163

Place Of Publication


  • New York

Abstract


  • The proliferation of all organisms depends on the coordination of enzymatic events within

    large multiprotein replisomes that duplicate chromosomes. Whereas the structure and function

    of many core replisome components have been clarified, the timing and order of molecular

    events during replication remains obscure. To better understand the replication

    mechanism, new methods must be developed that allow for the observation and characterization

    of short-lived states and dynamic events at single replication forks. Over the last

    decade, great progress has been made toward this goal with the development of novel

    DNA nanomanipulation and fluorescence imaging techniques allowing for the direct observation

    of replication-fork dynamics both reconstituted in vitro and in live cells. This article

    reviews these new single-molecule approaches and the revised understanding of replisome

    operation that has emerged.

Authors


  •   Duderstadt, Karl E. (external author)
  •   Reyes-Lamothe, Rodrigo (external author)
  •   van Oijen, Antoine M.
  •   Sherratt, David J. (external author)

Publication Date


  • 2013

Citation


  • Duderstadt, K. E., Reyes-Lamothe, R., van Oijen, A. M. & Sherratt, D. J. (2013). Replication-fork dynamics. In S. Bell, M. Mechali & M. L. Depamphilis (Eds.), DNA Replication: a Subject Collection from Cold Spring Harbor Perspectives in Biology (pp. 147-163). New York: Cold Spring Harbor Laboratory Press.

Scopus Eid


  • 2-s2.0-84891649417

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2279

Book Title


  • DNA Replication: a Subject Collection from Cold Spring Harbor Perspectives in Biology

Has Global Citation Frequency


Start Page


  • 147

End Page


  • 163

Place Of Publication


  • New York