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Single-molecule enzymology

Chapter


Abstract


  • Understanding how enzymes function requires a thorough characterization of

    enzymatic dynamics. Traditional enzymatic assays average over an ensemble of molecules,

    making it difficult to detect reaction intermediates and conformational fluctuations of the

    enzyme. These problems can be overcome by observing enzymes functioning in real time

    on the single-molecule level. This chapter describes recent research efforts to measure singlemolecule

    enzyme kinetics and observe the structural dynamics of enzymes and discusses new

    approaches to study multiprotein complexes on the single-molecule level.

Publication Date


  • 2009

Citation


  • Loparo, J. J. & van Oijen, A. M. (2009). Single-molecule enzymology. In P. Hinterdorfer & A. M. van Oijen (Eds.), Handbook of Single-Molecule Biophysics (pp. 165-182). Dordrecht: Springer.

International Standard Book Number (isbn) 13


  • 9780387764962

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2278

Book Title


  • Handbook of Single-Molecule Biophysics

Start Page


  • 165

End Page


  • 182

Place Of Publication


  • Dordrecht

Abstract


  • Understanding how enzymes function requires a thorough characterization of

    enzymatic dynamics. Traditional enzymatic assays average over an ensemble of molecules,

    making it difficult to detect reaction intermediates and conformational fluctuations of the

    enzyme. These problems can be overcome by observing enzymes functioning in real time

    on the single-molecule level. This chapter describes recent research efforts to measure singlemolecule

    enzyme kinetics and observe the structural dynamics of enzymes and discusses new

    approaches to study multiprotein complexes on the single-molecule level.

Publication Date


  • 2009

Citation


  • Loparo, J. J. & van Oijen, A. M. (2009). Single-molecule enzymology. In P. Hinterdorfer & A. M. van Oijen (Eds.), Handbook of Single-Molecule Biophysics (pp. 165-182). Dordrecht: Springer.

International Standard Book Number (isbn) 13


  • 9780387764962

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2278

Book Title


  • Handbook of Single-Molecule Biophysics

Start Page


  • 165

End Page


  • 182

Place Of Publication


  • Dordrecht